Discrimination of Genetically Very Close Accessions of Sweet Orange (Citrus sinensis L. Osbeck) by Laser-Induced Breakdown Spectroscopy (LIBS)
| Autor: | Thiago Massaiti, Aida Bebeachibuli Magalhães, Valdenice M. Novelli, A. C. Ranulfi, Giorgio S. Senesi, Bruno S. Marangoni, Mariângela Cristofani-Yaly, P. R. V. Boas, Ednaldo José Ferreira, Marina Nery da Silva, Débora Marcondes Bastos Pereira Milori |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Canopy
Citrus Pharmaceutical Science sweet orange accessions 02 engineering and technology Orange (colour) Biology 01 natural sciences Article Analytical Chemistry QD241-441 elemental concentration Drug Discovery Partial least squares regression Laser-induced breakdown spectroscopy Physical and Theoretical Chemistry Principal Component Analysis Lasers Spectrum Analysis 010401 analytical chemistry Organic Chemistry 021001 nanoscience & nanotechnology ESPECTROSCOPIA classification via regression (CVR) 0104 chemical sciences Horticulture laser-induced breakdown spectroscopy Chemistry (miscellaneous) Genetic marker partial least square regression (PLSR) Principal component analysis Molecular Medicine 0210 nano-technology Rootstock Citrus × sinensis |
| Zdroj: | Molecules Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Molecules (Basel, Online) 26 (2021): 3092-1–3092-19. doi:10.3390/molecules26113092 info:cnr-pdr/source/autori:Magalhaes A.B.; Senesi G.S.; Ranulfi A.; Massaiti T.; Marangoni B.S.; Da Silva M.N.; Villas Boas P.R.; Ferreira E.; Novelli V.M.; Cristofani-Yaly M.; Milori D.M.B.P./titolo:Discrimination of genetically very close accessions of sweet orange (Citrus sinensis l. osbeck) by laser-induced breakdown spectroscopy (libs)/doi:10.3390%2Fmolecules26113092/rivista:Molecules (Basel, Online)/anno:2021/pagina_da:3092-1/pagina_a:3092-19/intervallo_pagine:3092-1–3092-19/volume:26 Molecules, Vol 26, Iss 3092, p 3092 (2021) Volume 26 Issue 11 |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules26113092 |
| Popis: | The correct recognition of sweet orange (Citrus sinensis L. Osbeck) variety accessions at the nursery stage of growth is a challenge for the productive sector as they do not show any difference in phenotype traits. Furthermore, there is no DNA marker able to distinguish orange accessions within a variety due to their narrow genetic trace. As different combinations of canopy and rootstock affect the uptake of elements from soil, each accession features a typical elemental concentration in the leaves. Thus, the main aim of this work was to analyze two sets of ten different accessions of very close genetic characters of three varieties of fresh citrus leaves at the nursery stage of growth by measuring the differences in elemental concentration by laser-induced breakdown spectroscopy (LIBS). The accessions were discriminated by both principal component analysis (PCA) and a classifier based on the combination of classification via regression (CVR) and partial least square regression (PLSR) models, which used the elemental concentrations measured by LIBS as input data. A correct classification of 95.1% and 80.96% was achieved, respectively, for set 1 and set 2. These results showed that LIBS is a valuable technique to discriminate among citrus accessions, which can be applied in the productive sector as an excellent cost–benefit tool in citrus breeding programs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |