Association of Urinary Laminin G-Like 3 and Free K Light Chains with Disease Activity and Histological Injury in IgA Nephropathy
| Autor: | Leonarda Varraso, Giuseppe Grandaliano, Grazia Vocino, Maria Teresa Rocchetti, Massimo Papale, Salvatore Di Paolo, Anna Maria Di Palma, Eustacchio Montemurno, Loreto Gesualdo, Ida Valentina Suriano, Anna Maria d’Apollo |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Proteomics Nephrology medicine.medical_specialty Pathology Epidemiology Biopsy Urinary system Blotting Western Down-Regulation Urine Critical Care and Intensive Care Medicine Severity of Illness Index Gastroenterology Nephropathy Immunoglobulin kappa-Chains Nephelometry and Turbidimetry Predictive Value of Tests Internal medicine medicine Humans Analysis of Variance Transplantation medicine.diagnostic_test business.industry Case-control study Reproducibility of Results Glomerulonephritis IGA Glomerulonephritis Original Articles Middle Aged medicine.disease Immunohistochemistry Peptide Fragments Case-Control Studies Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Linear Models Female Laminin Renal biopsy business Biomarkers Heparan Sulfate Proteoglycans |
| Zdroj: | Clinical Journal of the American Society of Nephrology. 8:1115-1125 |
| ISSN: | 1555-9041 |
| Popis: | Summary Background and objectives IgA nephropathy has variable clinical presentation and progression. Its definitive diagnosis and prognosis require renal biopsy. The identification of new biomarkers allowing noninvasive diagnosis and monitoring of disease activity would be advantageous. This study analyzed the urine proteome of IgA nephropathy patients at an early stage of disease. Design, setting, participants, & measurements Urine from 49 IgA nephropathy patients, 42 CKD patients, and 40 healthy individuals was analyzed by surface-enhanced laser desorption/ionization time of flight/mass spectrometry. Differentially excreted proteins were identified by matrix-enhanced laser desorption/ionization time of flight/mass spectrometry, confirmed by immunologic methods, and validated in an independent set of patients (14 IgA nephropathy and 24 CKD). All patients were recruited at the Division of Nephrology of the University of Foggia from January of 2005 to March of 2007. Results Two proteins, with 21,598 and 23,458 m/z, were significantly decreased in IgA nephropathy and identified as Perlecan laminin G-like 3 peptide and Ig κ light chains, respectively. Western blot analysis confirmed the lower urinary excretion of laminin G-like 3 in IgA nephropathy patients compared with CKD patients and healthy individuals. Immunonephelometry analysis confirmed the lower urinary excretion of free κ light chains in IgA nephropathy patients compared with CKD patients and healthy individuals. Immunohistochemistry analysis justified the urinary excretion profile of such proteins in IgA nephropathy. Finally, urinary free κ light chains and laminin G-like 3 concentration inversely correlated with severity of clinical and histologic features of our IgA nephropathy cohort. Conclusions Laminin G-like 3 and free κ light chains can contribute to the noninvasive assessment of IgA nephropathy disease activity. |
| Databáze: | OpenAIRE |
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