NO is not sufficient to explain maximal cytotoxicity of tumoricidal macrophages against an NO-sensitive cell line
| Autor: | R. B. Mateo, Jonathan S. Reichner, Jorge E. Albina |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Cytotoxicity
Immunologic Lipopolysaccharides Male Lipopolysaccharide Vasodilator Agents Immunology Cell Mast-Cell Sarcoma Lymphocyte proliferation Biology S-Nitroso-N-Acetylpenicillamine Lymphocyte Activation Nitric Oxide Sensitivity and Specificity Nitric oxide chemistry.chemical_compound Mice medicine Immunology and Allergy Cytotoxic T cell Macrophage Animals Humans Cytotoxicity Cells Cultured Cell Death Effector Tumor Necrosis Factor-alpha Macrophages Penicillamine Cell Biology Macrophage Activation Rats Inbred F344 Cell biology Rats medicine.anatomical_structure chemistry Wounds and Injuries Cattle Nitric Oxide Synthase Reactive Oxygen Species |
| Zdroj: | Journal of leukocyte biology. 60(2) |
| ISSN: | 0741-5400 |
| Popis: | Nitric oxide (NO) is a macrophage cytotoxic effector. Results presented here, however, demonstrate that NO does not fully explain macrophage cytotoxicity against NO-sensitive cells because (1) inhibition of NO production by activated macrophages reduces, not eliminates, cytotoxicity; (2) NO produced chemically in amounts equimolar to those released from macrophages fails to lyse P815 cells; and (3) macrophages isolated from wounds 10 days after injury generate NO just as tumoricidal activated macrophages but are not tumor cytotoxic. The noncytotoxic nature of these wound-derived macrophages is not explained by the release of inactive forms of NO, because they suppress lymphocyte proliferation in an NO-dependent manner, nor by the production of cytoprotective molecules, because their addition to activated macrophage–tumor cell cocultures does not quench cytotoxicity. Interestingly, cytotoxicity can be aroused in day 10 wound-derived macrophages by culture with lipopolysaccharide, and macrophages harvested earlier in the development of the wound are cytotoxic. By generating NO but not killing an NO-sensitive cell, day 10 wound-derived macrophages demonstrate that NO production is not sufficient to account for the killing of an NO-sensitive tumor by macrophages. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text