The usefulness of a single measurement of insulin-like growth factor-1 as a predictor of embryo yield and pregnancy rates in a bovine MOET program
| Autor: | Mark A. Crowe, Robert Smith, Peter J. Cripps, M. Newman, M. F. Christie, Miguel A. Velazquez, Hilary Dobson |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Ovulation
medicine.medical_specialty animal structures media_common.quotation_subject medicine.medical_treatment Fatty Acids Nonesterified Biology Andrology Insulin-like growth factor NEFA Ovulation Induction Food Animals Pregnancy Internal medicine medicine Animals Insulin Urea Insulin-Like Growth Factor I Small Animals Insemination Artificial media_common Estrous cycle 3-Hydroxybutyric Acid Equine Artificial insemination Embryo Embryo Transfer Embryo Mammalian medicine.disease Tissue Donors Embryo transfer Cholesterol Endocrinology Tissue and Organ Harvesting Cattle Female Animal Science and Zoology Follicle Stimulating Hormone Biomarkers |
| Zdroj: | Theriogenology. 64:1977-1994 |
| ISSN: | 0093-691X |
| DOI: | 10.1016/j.theriogenology.2005.05.001 |
| Popis: | The objective was to determine if a single measurement of plasma insulin-like growth factor-1 (IGF-1) could predict the number of viable embryos obtained from donors and the likelihood of pregnancy in recipients in multiple ovulation and embryo transfer (MOET) programs in cattle. The embryo yields from 101 embryo recoveries were examined in maiden Holstein heifers (n=75) and multiparous Holstein cows (lactating cows n=20, dry cows n=6). Donors were super stimulated with FSH and embryo recovery was done non-surgically 7 days after artificial insemination. Embryos were classified according to the IETS criteria. Pregnancy rates in 100 maiden Holstein heifer recipients were analysed. Recipients were on day 7+/-1 of the estrous cycle at transfer. Pregnancy diagnosis was carried out at day 30 (PD 30) and rechecked at day 60 (PD 60) after transfer. Blood samples from coccygeal vessels taken at the time of embryo recovery (donors) and transfer (recipients) were analysed for IGF-1, insulin, beta-hydroxybutyrate (beta-OHB), non-esterified fatty acids (NEFA), urea and cholesterol. There was a negative correlation between the number of viable embryos and insulin (r=-0.33, P=0.025) in donor heifers. In donor cows, the number of viable embryos was correlated with IGF-1 (r=0.43, P=0.028) and cholesterol (r=-0.43, P=0.027). In recipients, PD30 and PD 60 were not affected by any of the circulating parameters analysed. Insulin, IGF-1 and cholesterol only explained 8.9, 13.9 and 15.8% of the variation in the production of viable embryos, respectively. Several factors affect MOET programs and under the circumstances of the present study the usefulness of hormonal and metabolic profiles as predictors of the outcome of this biotechnology was limited. |
| Databáze: | OpenAIRE |
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