Effect of Siam weed extract and its bioactive component scutellarein tetramethyl ether on anti-inflammatory activity through NF-κB pathway
| Autor: | Wandee Gritsanapan, Xiaobo Zhang, Suchitra Thongpraditchote, Hataichanok Pandith, Seung Joon Baek, Yuvadee Wongkrajang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
medicine.drug_class
medicine.medical_treatment Anti-Inflammatory Agents Nitric Oxide Synthase Type II IκB kinase Pharmacology Nitric Oxide Dinoprostone Anti-inflammatory Cell Line Mice chemistry.chemical_compound Isosakuranetin Western blot Drug Discovery medicine Animals RNA Messenger Apigenin Cell Proliferation biology medicine.diagnostic_test Interleukin-6 Plant Extracts Tumor Necrosis Factor-alpha Chromolaena NF-kappa B Biological activity NF-κB Plant Leaves Nitric oxide synthase chemistry Cyclooxygenase 2 biology.protein Prostaglandin E |
| Zdroj: | Journal of Ethnopharmacology. 147:434-441 |
| ISSN: | 0378-8741 |
| Popis: | Ethnopharmacological relevance Siam weed ( Chromolaena odorata (L.) King and Robinson) is a medicinal herb used for wound healing and inflammation-related diseases. Aim of the study In this study, we evaluated the molecular mechanism by which Siam weed extract (SWE) and its bioactive components, scutellarein tetramethyl ether (scu), stigmasterol, and isosakuranetin affect anti-inflammatory activity. Materials and methods The expression of several inflammatory proteins in RAW 264.7 (murine) macrophages was assessed by Western blot and reverse transcription-polymerase chain reaction (RT-PCR). Biochemical assays including prostaglandin E 2 (PGE 2 ) and nitric-oxide (NO) quantification were performed. Luciferase promoter activity and immunocytochemistry of Nuclear factor-κB (NF-κB) were investigated. Results Cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) are critical pro-inflammatory proteins. The level of protein and mRNA expression of these enzymes induced by lipopolysaccharide (LPS) was dramatically suppressed by treatment with SWE, scu, or stigmasterol compounds in a dose-dependent manner. They also reduced PGE 2 and NO release. We further analyzed the NF-κB pathway and found that the scu compound suppressed IκB kinase complex alpha/beta (IKK α / β ) and Inhibitory-kappa-B-alpha (IκB α ), thereby suppressing COX-2 and iNOS expression. Conclusion This is the first report of the anti-inflammatory molecular mechanism in SWE and/or its bioactive component scu, indicating alteration NF-κB pathway and further providing potential uses in the treatment of inflammatory-related diseases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect