Anti-viral activity of hairpin ribozyme directed against HBV core regionin vitro
| Autor: | Youxin Jin, Jusheng Lin, Xin-Juan Kong, Xiaojun Wu, Ning Wang, E Cao, Na Xie, Yu-Hu Song, Nan-Zhi Liu |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Hepatitis B virus
Transcription Genetic Molecular Sequence Data Virus Replication medicine.disease_cause Antiviral Agents Small hairpin RNA Management of Technology and Innovation Escherichia coli medicine T7 RNA polymerase RNA Catalytic Base Sequence biology Gene Transfer Techniques Ribozyme RNA Hepatitis B Core Antigens Molecular biology Enzyme Activation biology.protein RNA Viral Mammalian CPEB3 ribozyme Hairpin ribozyme VS ribozyme medicine.drug |
| Zdroj: | Journal of Tongji Medical University. 21:219-221 |
| ISSN: | 0257-716X |
| DOI: | 10.1007/bf02886434 |
| Popis: | To study the preparation and cleavage of hairpin ribozyme (HpRz) directed against the transcript of HBV core region in vitro, HRz gene designed by computer targeting the transcript of HBV core gene was cloned into the vector p1.5 between 5'-cis-Rz and 3'-cis-Rz. 32P-labeled HpRz transcript proved whether the vector fit for the preparation of hairpin ribozyme. 32P-labeled pKC transcript containing HBV core region as targets-RNA was transcribed by using T7 RNA polymerase and purified by PAGE. Cold HpRz transcript was incubated with 32P-labeled target-RNAs under different conditions and radioautographed after denaturing polyacrylamide gel electrophoresis. The results showed that HpRz had the ability of cleavage at 37 degrees C and 12 mmol/L MgCl2 and the design of ribozyme was correct. It is concluded that HpRz prepared in vitro possesses specific catalytic activity, indicating that it is possible for HpRz to intracellularly inhibit the replication of HBV. It may be developed into a nucleic acid drug in the treatment of hepatitis B in the future. |
| Databáze: | OpenAIRE |
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