Magnetic resonance imaging at 1.5 T with immunospecific contrast agent in vitro and in vivo in a xenotransplant model
| Autor: | L. E. Derchi, Marina Fabbi, D. de Totero, Gabriella Baio, C. E. Neumaier, Silvano Ferrini, M. Cilli |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Ultrasmall particle iron oxide
Lymphoma Iron Transplantation Heterologous Biophysics Contrast Media Tumor cells Magnetic resonance imaging anti-CD20 antibodies Antibodies Monoclonal Murine-Derived Mice Magnetite Nanoparticles Drug Delivery Systems Nuclear magnetic resonance In vivo Cell Line Tumor medicine Animals Radiology Nuclear Medicine and imaging Radiological and Ultrasound Technology medicine.diagnostic_test Chemistry Antibodies Monoclonal Dextrans Oxides Antigens CD20 Image Enhancement Ferrosoferric Oxide In vitro Transplantation Disease Models Animal Monoclonal Rituximab |
| Popis: | Demonstrating the feasibility of magnetic resonance imaging (MRI) at 1.5 T of ultrasmall particle iron oxide (USPIO)-antibody bound to tumor cells in vitro and in a murine xenotransplant model.Human D430B cells or Raji Burkitt lymphoma cells were incubated in vitro with different amounts of commercially available USPIO-anti-CD20 antibodies and cell pellets were stratified in a test tube. For in vivo studies, D430B cells and Raji lymphoma cells were inoculated subcutaneously in immunodeficient mice. MRI at 1.5 T was performed with T1-weighted three-dimensional fast field echo sequences (17/4.6/13 degrees ) and T2-weighted three-dimensional fast-field echo sequences (50/12/7 degrees ). For in vivo studies MRI was performed before and 24 h after USPIO-anti-CD20 administration.USPIO-anti-CD20-treated D430B cells, showed a dose-dependent decrease in signal intensity (SI) on T2*-weighted images and SI enhancement on T1-weighted images in vitro. Raji cells showed lower SI changes, in accordance to the fivefold lower expression of CD20 on Raji with respect to D430B cells. In vivo 24 h after USPIO-anti-CD20 administration, both tumors showed an inhomogeneous decrease of SI on T2*-weighted images and SI enhancement on T1-weighted images.MRI at 1.5 T is able to detect USPIO-antibody conjugates targeting a tumor-associated antigen in vitro and in vivo. |
| Databáze: | OpenAIRE |
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