An anti-human immunoglobulin M monoclonal antibody for detection of antibodies toToxoplasma gondii
| Autor: | M. Garcia-Gonzalez, G. Desmonts, H. Thoannes, J. M. Pinon, J. Kadouche, P. Thulliez, Ph. Pouletty |
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| Rok vydání: | 1984 |
| Předmět: |
Microbiology (medical)
medicine.drug_class Fluorescent Antibody Technique Monoclonal antibody Microbiology Toxoplasmosis Congenital Antigen parasitic diseases Human Immunoglobulin M medicine Humans Direct fluorescent antibody Immunosorbent Techniques biology Immunoglobulin mu-Chains Chemistry Antibodies Monoclonal Infant Radioimmunoassay General Medicine IgM binding Virology Molecular biology Antibodies Anti-Idiotypic Infectious Diseases Immunoglobulin M Polyclonal antibodies biology.protein Antibody Toxoplasma Toxoplasmosis |
| Zdroj: | European Journal of Clinical Microbiology. 3:510-515 |
| ISSN: | 1435-4373 0722-2211 |
| Popis: | An anti-human mu-chain monoclonal antibody, Tibi 82, was produced and tested for specificity by radioimmunoassay. Its reliability in detecting IgM antibodies to Toxoplasma gondii was tested by two reverse immunosorbent methods (IgM-ISAGA and IgM-SPIHA) and the IgM fluorescent antibody test (IgM-IFA) on 400 sera. Whereas the results obtained with Tibi 82 and with two polyclonal reagents were highly correlated, the third commercial polyclonal reagent provided many false negative results. By standardizing IgM binding, Tibi 82 allowed the comparison of IgM-ISAGA with IgM-SPIHA on 100 sera: 17% of the sera tested showed discrepancies due to the different toxoplasma antigens used. Although Tibi 82 facilitated the reading of results and enhanced sensitivity and specificity of the double-sandwich IgM-IFA method, the latter was still less sensitive than IgM-ISAGA with Tibi 82. Tests with the monoclonal antibody were consistently superior to tests with polyclonal antibodies. |
| Databáze: | OpenAIRE |
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