Molecular epidemiology of Pseudomonas cepacia determined by polymerase chain reaction ribotyping
Autor: | T L Stull, Thomas D. Edlind, J R Kostman, John J. LiPuma |
---|---|
Rok vydání: | 1992 |
Předmět: |
DNA
Bacterial Microbiology (medical) Molecular Sequence Data Burkholderia cepacia Polymerase Chain Reaction law.invention Microbiology chemistry.chemical_compound Ribotyping law Humans Gene Polymerase chain reaction Genetics Polymorphism Genetic Base Sequence Molecular epidemiology biology Pseudomonas Ribosomal RNA biology.organism_classification Bacterial Typing Techniques RNA Bacterial chemistry Evaluation Studies as Topic RNA Ribosomal Restriction fragment length polymorphism Epidemiologic Methods DNA Research Article |
Zdroj: | Journal of Clinical Microbiology. 30:2084-2087 |
ISSN: | 1098-660X 0095-1137 |
Popis: | Traditional ribotyping detects genomic restriction fragment length polymorphisms by probing chromosomal DNA with rRNA. Although it is a powerful method for determining the molecular epidemiology of bacterial pathogens, technical difficulties limit its application. As an alternative, polymorphisms were sought in the 16S-23S spacer regions of bacterial rRNA genes by use of the polymerase chain reaction (PCR). Chromosomal DNA from isolates of Pseudomonas cepacia was used as a template in the PCR with oligonucleotide primers complementary to highly conserved sequences flanking the spacer regions of the rRNA genes. Length polymorphisms in the amplified DNA distinguished unrelated isolates of P. cepacia. Isolates of P. cepacia previously implicated in person-to-person transmission were shown to have identical amplification patterns. These data demonstrate the utility of this new PCR ribotyping method for determining the molecular epidemiology of bacterial species. |
Databáze: | OpenAIRE |
Externí odkaz: |