Monoclonal antibodies which identify a genus-specific Listeria antigen
| Autor: | R J Durham, M C Plank, B T Butman, J. A. Mattingly |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
medicine.drug_class
Listeria Enzyme-Linked Immunosorbent Assay Biology Cross Reactions Monoclonal antibody medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Antigen Listeria monocytogenes Western blot Species Specificity medicine Animals Immunoassay Antigens Bacterial Hybridomas Ecology medicine.diagnostic_test Antibodies Monoclonal biology.organism_classification Isotype Molecular biology Antibodies Bacterial Molecular Weight biology.protein Antibody Food Science Biotechnology Research Article |
| Zdroj: | Applied and environmental microbiology. 54(6) |
| ISSN: | 0099-2240 |
| Popis: | Fifteen murine monoclonal antibodies (MAbs) which react specifically with a protein antigen found in all species of Listeria were developed and characterized. These MAbs were tested extensively by both enzyme-linked immunosorbent assay and Western blot (immunoblot) analyses for cross-reaction with non-Listeria organisms, such as Staphylococcus, Streptococcus, Citrobacter, Pseudomonas, and Salmonella species, and were found to be nonreactive. The genus-specific antigen was identified as a heat-stable protein with a molecular weight in the range of 30,000 to 38,000 (under both reducing and nonreducing conditions), depending on the species of Listeria tested. In Listeria monocytogenes, L. innocua, L. ivanovii, and L. seeligeri the antigen has a molecular weight of approximately 30,000 to 34,000. In L. grayi and L. murrayi it has a molecular weight of approximately 35,000 to 38,000. In addition, several of the MAbs recognize lower-molecular-weight protein bands. There appear to be at least two groups of Listeria-specific MAbs based upon isotype and results of enzyme-linked immunosorbent assay and Western blot analyses. These MAbs have proven to be useful in the development of a diagnostic assay for Listeria species in food products. |
| Databáze: | OpenAIRE |
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