Cloning and expression of Staphylococcus aureus plasmid-mediated quaternary ammonium resistance in Escherichia coli
| Autor: | B. R. Lyon, Matthew T. Gillespie, J. W. May, Ronald A. Skurray, J M Tennent |
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| Jazyk: | angličtina |
| Rok vydání: | 1985 |
| Předmět: |
DNA
Bacterial Staphylococcus aureus R Factors Biology Molecular cloning medicine.disease_cause Microbiology Restriction map Plasmid medicine Escherichia coli Pharmacology (medical) Cloning Molecular Pharmacology Genetic transfer Chromosome Mapping Chromosomes Bacterial Molecular biology PBR322 Quaternary Ammonium Compounds Restriction enzyme Infectious Diseases Subcloning Transformation Bacterial Research Article |
| Popis: | The Staphylococcus aureus plasmid pSK1 carries Tn4001, a 4.7-kilobase (kb) transposon which specifies resistance to gentamicin, tobramycin, and kanamycin. In addition, pSK1 mediates resistance to trimethoprim and linked resistance to ethidium bromide (Ebr) and to quaternary ammonium compounds (Qar). Restriction endonuclease analysis of pSK1 and a deleted derivative of pSK1 revealed that the gene(s) responsible for Ebr Qar lies within a 5.2-kb HindIII fragment. This fragment has been cloned into the Escherichia coli plasmid vector pBR322, and transformants of an E. coli K-12 strain exhibited Ebr Qar. Subcloning of the 5.2-kb insert, combined with data from electron microscopic analysis of deleted derivatives of pSK1, located the Ebr Qar determinant(s) on a 2.3-kb segment of pSK1 DNA. |
| Databáze: | OpenAIRE |
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