Cross‐reactivity of the IDEXX Legiolert method with other Gram‐negative bacteria and waterborne pathogens leads to false‐positive assay results
Autor: | Marisa B Hirsh, Janet E. Stout, Sue M. Mietzner, Julianne L. Baron, John D. Rihs |
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Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
Legionella Cross Reactions medicine.disease_cause 01 natural sciences Applied Microbiology and Biotechnology Legionella pneumophila Microbiology 03 medical and health sciences Water Supply 010608 biotechnology medicine Humans False Positive Reactions Elizabethkingia meningoseptica 0303 health sciences biology 030306 microbiology Pseudomonas aeruginosa Bacterial pneumonia Water biology.organism_classification medicine.disease Proteus mirabilis Serratia marcescens Legionnaires' Disease Water Microbiology Bacteria Environmental Monitoring |
Zdroj: | Letters in Applied Microbiology. 72:750-756 |
ISSN: | 1472-765X 0266-8254 |
Popis: | Legionella species are the causative agent of Legionnaires' disease, a potentially fatal bacterial pneumonia. New regulations and standards have prioritized the development of water safety plans to minimize the growth and spread of Legionella species in buildings. To determine the presence and type of Legionella in a water system, microbiological culturing is the gold standard method. However, recently new methodologies have been developed that claim to be sensitive and specific for Legionella at the genus or L. pneumophila at the species level. Published and anecdotal reports suggest that one of these newer culture-based, enzyme-substrate methods, the IDEXX Legiolert test, may exhibit false positivity with other microbes common to water sources. We experimentally evaluated the IDEXX Legiolert method using these other waterborne bacteria including Elizabethkingia meningoseptica, Pseudomonas aeruginosa, Proteus mirabilis and Serratia marcescens at real-world environmental concentrations. We saw false-positive results for the Legiolert test with several of these organisms, at sample concentrations as low as 60 CFU per ml. False-positive Legionella results can trigger costly remediation and water-use restrictions, that may be implemented while waiting for additional, confirmatory microbiological testing that could, in this case, yield no L. pneumophila. |
Databáze: | OpenAIRE |
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