Mining gut microbiome oligopeptides by functional metaproteome display
| Autor: | Stefan Dübel, Jonas Zantow, Michael Hust, Sarah Just, Sigrid Kisling, Thomas Clavel, Ilias Lagkouvardos, Patricia Lepage |
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| Přispěvatelé: | Clavel, Thomas, Hust, Michael, Technical University Braunschweig, ZIEL – Institute for Food and Health, Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Phage display Proteome [SDV]Life Sciences [q-bio] 030106 microbiology Mice Transgenic Computational biology Bioinformatics Article Mice 03 medical and health sciences Immune system Antigen Peptide Library Animals Peptide library Multidisciplinary biology Gastrointestinal Microbiome Ileitis ddc 3. Good health 030104 developmental biology Metagenomics Chronic Disease biology.protein Metagenome Antibody Oligopeptides |
| Zdroj: | Scientific Reports (6), 1-13. (2016) Scientific Reports Scientific Reports, Nature Publishing Group, 2016, 6, pp.1-13. ⟨10.1038/srep34337⟩ |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep34337 |
| Popis: | Pathogen infections, autoimmune diseases and chronic inflammatory disorders are associated with systemic antibody responses from the host immune system. Disease-specific antibodies can be important serum biomarkers, but the identification of antigens associated with specific immune reactions is challenging, in particular if complex communities of microorganisms are involved in the disease progression. Despite promising new diagnostic opportunities, the discovery of these serological markers becomes more difficult with increasing complexity of microbial communities. In the present work, we used a metagenomic M13 phage display approach to select immunogenic oligopeptides from the gut microbiome of transgenic mice suffering from chronic ileitis. We constructed three individual metaproteome phage display libraries with a library size of approximately 107 clones each. Using serum antibodies, we selected and validated three oligopeptides that induced specific antibody responses in the mouse model. This proof-of-concept study provides the first successful application of functional metaproteome display for the study of protein-protein interactions and the discovery of potential disease biomarkers. |
| Databáze: | OpenAIRE |
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