Molecular characterization of bovine leukemia virus reveals existence of genotype 4 in Chinese dairy cattle
| Autor: | Maoli Dong, Yalan Peng, Lina Chen, Yi Yang, Xiaoli Hao, Wenjiang Huang, Aijian Qin, Zaicheng Gong, Shaobin Shang, Zhangping Yang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
China Genotype animal diseases viruses Short Report Cattle Diseases Biology Envelope gene Genes env Bovine leukemia virus Genetic diversity Leucosis lcsh:Infectious and parasitic diseases Evolution Molecular 03 medical and health sciences 0302 clinical medicine Viral Envelope Proteins Virology Leukemia Virus Bovine Animals lcsh:RC109-216 Genetic variability Gene Phylogeny Phylogenetic tree virus diseases Genetic Variation Sequence Analysis DNA Enzootic Bovine Leukosis biology.organism_classification Molecular characterization Dairying 030104 developmental biology Infectious Diseases GenBank Mutation Enzootic 030211 gastroenterology & hepatology Cattle Female |
| Zdroj: | Virology Journal Virology Journal, Vol 16, Iss 1, Pp 1-7 (2019) |
| ISSN: | 1743-422X |
| Popis: | Bovine leukemia virus (BLV) causes enzootic bovine leucosis and is widely spread worldwide, except several European countries, Australia and New Zealand. Although BLV is highly prevalent in China, information about the genetic diversity and evolutionary dynamics of BLV among Chinese dairy herds is still lacking. To determine the genetic variability of BLV, 219 cows from four cities of Ningxia province of China were screened for BLV infection by fluorescence resonance energy transfer (FRET)-PCR and sequencing, 16 selected positive samples were subjected to molecular characterization. Phylogenetic analysis using the neighbor-joining (NJ) method on complete sequences of envelope (env) gene of BLV obtained from China and those available in GenBank (representing BLV genotypes 1–10) revealed that those Chinese strains belonged to genotypes 4 and 6. Totally, 23 mutations were identified and 16 of them were determined to be unique mutations among Chinese strains. Alignment of the deduced amino acid sequences demonstrated six mutations in glycoprotein 51 (gp51) and three mutations in glycoprotein 30 (gp30) located in the identified neutralizing domain (ND), CD8+ T cell epitope, E-epitope, B-epitope, gp51N12 and cytoplasmic domain of transmembrane protein. This study reported for the first time the BLV genotype 4 in China, and further studies are warranted to compare its immunogenicity and pathogenicity with other BLV genotypes. Electronic supplementary material The online version of this article (10.1186/s12985-019-1207-8) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |