Interesterification of Soybean Oil with Propylene Glycol in Supercritical Carbon Dioxide and Analysis by NMR Spectroscopy
| Autor: | Nazanin Vafaei, Martin G. Scanlon, Curtis Rempel, Michael N. A. Eskin, Peter B. Jones |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Magnetic Resonance Spectroscopy food.ingredient Interesterified fat Glyceride Bioengineering 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Catalysis Soybean oil Diglycerides Fungal Proteins Glycols Industrial Microbiology food 010608 biotechnology Organic chemistry Molecular Biology Triglycerides chemistry.chemical_classification Supercritical carbon dioxide Esterification 010405 organic chemistry Fatty Acids Lipase General Medicine Nuclear magnetic resonance spectroscopy Carbon Dioxide Carbon-13 NMR Enzymes Immobilized Propylene Glycol Supercritical fluid Soybean Oil 0104 chemical sciences chemistry Fatty Acids Unsaturated Monoglycerides Gases Biotechnology Polyunsaturated fatty acid |
| Zdroj: | Applied Biochemistry and Biotechnology. 191:905-920 |
| ISSN: | 1559-0291 0273-2289 |
| DOI: | 10.1007/s12010-019-03200-0 |
| Popis: | The time course study of high monoester mixtures from soybean oil (HMMS) synthesis, as healthier alternatives to trans food products, in a supercritical CO2 (SCCO2) medium with and without enzyme, was investigated. Phosphorous nuclear magnetic resonance (31P-NMR) was used to quantify the absolute amount of partially esterified acylglycerols (PEGs). Carbon NMR was utilized to determine the type and position of the fatty acids (FAs) of HMMS. Enzyme and time significantly influenced the synthesis of 1-monoglycerides (1-MGs), 2-MGs, and 1,2-diglycerides (1,2-DGs) in this alcoholysis of soybean oil with 1,2-propanediol, based on high catalytic activity and operational stability of Novozym 435 in SCCO2 during short reaction time. Results suggest that 4 h is a suitable reaction time for this lipase-catalyzed interesterification (LIE) system for the synthesis of 2-MGs with a yield of 20%. The highest polyunsaturated fatty acid (PUFA) (65%) in the triglyceride (TG) of HMMS was produced after 4 h of reaction. After 6 h of reaction, a high level (20%) of saturated fatty acids (SFAs) was found in the TGs of HMMS, which were distributed between the sn-2 (5%) and sn-1, 3 (15%) positions. |
| Databáze: | OpenAIRE |
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