Ion-exclusion chromatographic separations of C1–C6 aliphatic carboxylic acids on a sulfonated styrene–divinylbenzene co-polymer resin column with 5-methylhexanoic acid as eluent
| Autor: | Kazutoku Ohta, Masayoshi Ohashi, Atsuya Towata |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Polymers
Carboxylic acid Static Electricity Ion chromatography Carboxylic Acids Sensitivity and Specificity Biochemistry Caproic Acid Analytical Chemistry Gel permeation chromatography chemistry.chemical_compound Column chromatography Caproates Benzoic acid chemistry.chemical_classification Chromatography Chemistry Organic Chemistry Sulfuric acid General Medicine Benzoic Acid Hydrogen-Ion Concentration Sulfuric Acids Chromatography Ion Exchange Polystyrenes Indicators and Reagents Sulfonic Acids Aliphatic compound |
| Zdroj: | Journal of Chromatography A. 997:107-116 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(03)00627-7 |
| Popis: | The application of C7 aliphatic carboxylic acids (heptanoic, 2-methylhexanoic, 5-methylhexanoic and 2,2-dimethyl-n-valeric acids) as eluents in ion-exclusion chromatography with conductimetric detection for C1-C6 aliphatic carboxylic acids (formic, acetic, propionic, isobutyric, butyric, isovaleric, valeric, isocaproic and caproic acids) was carried out using a highly sulfonated styrene-divinylbenzene co-polymer resin (TSKgel SCX) in the H+ form as a stationary phase. When using 0.05 mM sulfuric acid at pH 4.0 as the eluent, peak shapes of hydrophobic carboxylic acids (isovaleric, valeric, isocaproic and caproic acids) were tailed strongly. In contrast, when using 1 mM these C7 carboxylic acids at pH ca. 4 as the eluents, although system peaks (vacant peaks) corresponding to these C7 carboxylic acids appeared, peak shapes of these hydrophobic acids were improved drastically. Excellent simultaneous separation and relatively high sensitive conductimetric detection for these C1-C6 aliphatic carboxylic acids were achieved in 25 min on the TSKgel SCX column (150 x 6 mm I.D.) using 1 mM 5-methylhexanoic acid at pH 4.0 as the eluent. |
| Databáze: | OpenAIRE |
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