Evidence for a cytosolic precursor of chick embryo liver mitochondrial δ-aminolevulinate synthase
| Autor: | Brian K. May, J.D. Brooker, Iain A. Borthwick, Gopesh Srivastava, W.H. Elliott |
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| Rok vydání: | 1983 |
| Předmět: |
Biophysics
Mitochondria Liver Chick Embryo Mitochondrion Biology Biochemistry Cytosol Animals RNA Messenger Molecular Biology Enzyme Precursors Messenger RNA Cell-Free System ATP synthase Embryo Translation (biology) Cell Biology In vivo labelling δ aminolevulinate synthase Molecular Weight Kinetics Liver Polyribosomes Protein Biosynthesis biology.protein 5-Aminolevulinate Synthetase |
| Zdroj: | Biochemical and Biophysical Research Communications. 110:23-31 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(83)91254-8 |
| Popis: | Following the recent demonstration [Borthwick, I.A., Srivastava, G., Brooker, J.D., May, B.K. and Elliott, W.H. (1982) Eur. J. Biochem. in press] that chick embryo liver mitochondrial δ-aminolevulinate synthase has a minimum molecular weight of 68,000 (rather than the hitherto accepted value of 49,000), we have shown that the primary translation product of δ-aminolevulinate synthase mRNA is a protein of molecular weight 74,000. This protein has for the first time been shown to occur in the cytosol fraction of drug-treated chick embryo livers. This form does not occur in mitochondria nor does the smaller mitochondrial form occur in the cytosol. It is concluded that the 74,000 molecular weight protein is a precursor which is processed during transport into the mitochondria. In vivo labelling experiments are consistent with this conclusion. |
| Databáze: | OpenAIRE |
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