T cell-intrinsic TLR2 stimulation promotes IL-10 expression and suppressive activity by CD45RbHi T cells
| Autor: | Douglas M. Oswald, Janice C. Jun, Mark B. Jones, Edward S. Sim, Lori S.C. Kreisman, Amruth R. Jonnalagadda, Brian A. Cobb |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
CD4-Positive T-Lymphocytes Male Physiology lcsh:Medicine Immune Receptors Biochemistry T-Lymphocytes Regulatory White Blood Cells Spectrum Analysis Techniques Cell Signaling Animal Cells Immune Physiology Medicine and Health Sciences Membrane Receptor Signaling IL-2 receptor lcsh:Science Toll-like Receptors Cells Cultured Innate Immune System Multidisciplinary Immune System Proteins TCR signaling cascade Chemistry T Cells FOXP3 Signaling cascades Regulatory T cells Flow Cytometry Immune Receptor Signaling Cell biology Interleukin-10 Interleukin 10 medicine.anatomical_structure Spectrophotometry Cytokines Female Cytophotometry Cellular Types Research Article Signal Transduction T cell Immune Cells Immunology Receptors Antigen T-Cell chemical and pharmacologic phenomena Research and Analysis Methods 03 medical and health sciences Immune system medicine Animals Innate immune system Blood Cells lcsh:R Interleukin-2 Receptor alpha Subunit Biology and Life Sciences Proteins Cell Biology Molecular Development Toll-Like Receptor 2 T Cell Receptors Mice Inbred C57BL TLR2 030104 developmental biology Immune System Leukocyte Common Antigens lcsh:Q Memory T cell Developmental Biology |
| Zdroj: | PLoS ONE, Vol 12, Iss 7, p e0180688 (2017) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | While Toll-like receptors (TLRs) represent one of the best characterized innate immune pathways, evidence suggests that TLRs are not restricted to innate leukocytes and some epithelial cells, but are also expressed in T cells. Specifically, published evidence focusing on FoxP3+ regulatory T cells demonstrate that they express functional TLR2, which is already known among the TLR family for its association with immune suppression; however, little is known about the relationship between T cell-intrinsic TLR2 binding and cytokine production, T cell differentiation, or T cell receptor (TCR) stimulation. Here, we demonstrate that TCR and TLR2 co-stimulation provides a T cell-intrinsic signal which generates a dramatic, synergistic cytokine response dominated by IL-10. Importantly, the response was not seen in either CD4+CD25+ or CD4+FoxP3+ Tregs, yet resulted in the expansion of a suppressive CD4+CD25+CD62L-CD44+CD45Rbhi effector/memory T cell subset not typically associated with immune inhibition. This study reveals the striking ability of a prototypical innate immune receptor to trigger a potent and suppressive IL-10 response in effector/memory T cells, supporting the notion that TLR2 is a co-regulatory receptor on T cells. |
| Databáze: | OpenAIRE |
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