Perception of environmental polypeptides in C. elegans activates insulin/IGF signaling and alters lipid metabolism
| Autor: | Rojin Chitrakar, Joseph A. Dent, Ryan Baugh L, Amy K. Webster, Kaplan Rew |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0303 health sciences
genetic structures Insulin medicine.medical_treatment fungi Lipid metabolism Metabolism Peptide secretion Biology behavioral disciplines and activities Cell biology 03 medical and health sciences 0302 clinical medicine Gene expression medicine Ingestion sense organs Gene Developmental biology 030217 neurology & neurosurgery psychological phenomena and processes 030304 developmental biology |
| DOI: | 10.1101/341883 |
| Popis: | Food perception affects animal physiology in complex ways. We uncoupled the effects of food perception and ingestion in the roundwormC. elegans. Perception was not sufficient to promote development, but larvae exposed to food without ingestion failed to develop upon return to normal culture conditions. Inhibition of gene expression during perception rescued subsequent development, demonstrating the response to perception without feeding is deleterious. Perception altered DAF-16/FOXO localization, reflecting activation of insulin/IGF signaling (IIS). The insulin-like peptidedaf-28was specifically required, suggesting perception in chemosensory neurons directly regulates peptide secretion. Gene expression and Nile Red staining suggest that perception alters lipid metabolism. Environmental polypeptides are sensed by starved larvae and promote dauer diapause recovery. We conclude that polypeptides are perceived as a food-associated cue, initiating a signaling and gene regulatory cascade that alters metabolism in anticipation of feeding and development, but that this response is detrimental if feeding does not occur. |
| Databáze: | OpenAIRE |
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