Apoptosis and proliferation in breast cancer cells, culturedin vitro: effects of SERMs
| Autor: | I. Vermes, H. R. Franke, H. M. J. Werner |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Selective Estrogen Receptor Modulators
medicine.medical_specialty Norpregnenes medicine.drug_class Apoptosis Breast Neoplasms Medroxyprogesterone Acetate Tibolone Breast cancer Cyclin D1 Cell Line Tumor Internal medicine Contraceptive Agents Female Tumor Cells Cultured medicine Humans Raloxifene Progesterone Cell Proliferation Estradiol business.industry Estrogen Replacement Therapy Obstetrics and Gynecology General Medicine medicine.disease Norethindrone Acetate Tamoxifen Endocrinology Estrogen Raloxifene Hydrochloride Cancer cell Dydrogesterone Cancer research Norethindrone business hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Climacteric. 8:294-299 |
| ISSN: | 1473-0804 1369-7137 |
| DOI: | 10.1080/13697130500197526 |
| Popis: | Selective estrogen receptor modulators (SERMs) decrease the risk of developing breast cancer. As an antagonistic effect, SERMs may aggravate or induce climacteric symptoms. Hormone therapy (HT) would be able to alleviate these symptoms. The present in vitro study tries to elucidate the effects of several HT preparations combined with SERMs on estrogen receptor-positive (ER +) (i.e. MCF-7 and T-47D) and -negative (ER-) (i.e. MDA-MB-231) human breast cancer cells in vitro.We performed experiments with various HT preparations (estradiol (E2)/E2 + progesterone/E2 + dihydrodydrogesterone /E2 + norethisterone acetate/E2 + medroxyprogesterone acetate/tibolone) in the concentration of 10(-6) mol/l together with SERMs (raloxifene or tamoxifen) added to different breast cancer cell lines in vitro. After an incubation period of 144 h, proliferation and apoptosis were measured. The first was measured by quantification of the expression of cyclin D1 mRNA, the latter by the Nicoletti method.This in vitro study clearly demonstrates differences in results if various HT preparations, combined with SERMs, are added to ER + and ER- breast cancer cell lines.Adding estradiol/progestogens in combination with a SERM to estrogen receptor-positive breast cancer cell lines does not obligatorily lead to proliferation of tumor cells. Not all progestogens act equally. |
| Databáze: | OpenAIRE |
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