Local peritoneal irrigation with intestinal alkaline phosphatase is protective against peritonitis in mice
| Autor: | H. Shaw Warren, Angela K. Moss, Farzad Ebrahimi, Elizabeth L. Hohmann, Golam Mostafa, Sundaram Ramasamy, Brishti Biswas, Nur Muhammad, Kathryn T. Chen, Richard A. Hodin, Halim Yammine, Sayeda Nasrin Alam, Madhu S. Malo |
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| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Intraperitoneal injection Peritonitis Inflammation Gastroenterology Sepsis Mice Internal medicine medicine Animals Peritoneal Lavage Survival rate Saline Lung business.industry medicine.disease Alkaline Phosphatase Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure Treatment Outcome Immunology Surgery Tumor necrosis factor alpha medicine.symptom business |
| Zdroj: | Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract. 15(5) |
| ISSN: | 1873-4626 |
| Popis: | The brush-border enzyme intestinal alkaline phosphatase (IAP) functions as a gut mucosal defense factor and detoxifies different toll-like receptor ligands. This study aimed to determine the therapeutic effects of locally administered calf IAP (cIAP) in a cecal ligation and puncture (CLP) model of polymicrobial sepsis. C57BL/6 mice underwent CLP followed by intraperitoneal injection of cIAP or normal saline. Blood leukocyte counts, levels of cytokines and liver enzymes, and lung myeloperoxidase activity were determined. Peritoneal lavage fluid (PLF) was assayed for neutrophil infiltration and both aerobic and anaerobic bacterial counts. After intraperitoneal injection, cIAP activity in PLF decreased 50% within 15 min with minimal activity evident at 4 h. Compared with irrigation with normal saline, cIAP irrigation increased the 7-day survival rate in mice undergoing CLP, with maximal effects seen at 25 units of cIAP (0% vs. 46% survival rate, respectively; p |
| Databáze: | OpenAIRE |
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