Curcumin Inhibits Viability of Clear Cell Renal Cell Carcinoma by Down-Regulating ADAMTS18 Gene Methylation though NF-κ B and AKT Signaling Pathway
| Autor: | Yi-Ji Peng, Ben Xu, Wei-Jie Zhu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
Curcumin 0211 other engineering and technologies 02 engineering and technology 030226 pharmacology & pharmacy 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine ADAMTS Proteins Cell Line Tumor 021105 building & construction Gene expression Humans Pharmacology (medical) Protein kinase B Carcinoma Renal Cell Akt/PKB signaling pathway NF-kappa B General Medicine Methylation DNA Methylation Molecular biology Kidney Neoplasms Complementary and alternative medicine chemistry DNA methylation Phosphorylation Matrix Metalloproteinase 2 Female Signal transduction Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | Chinese journal of integrative medicine. 28(5) |
| ISSN: | 1672-0415 |
| Popis: | To investigate the effect of curcumin on viability of clear cell renal cell carcinoma (ccRCC) and analyze its possible mechanism. In cell lines of A498 and 786-O, the effects of curcumin (1.25, 2.5, 5 and 10 μ mol/L) on the viability of ccRCC were analyzed at 24, 48 and 72 h by MTT assay. The protein expression levels of ADAMTS18 gene, p65, phosphorylation p65 (pp65), AKT, phosphorylation AKT (pAKT) and matrix metallopeptidase 2 (MMP-2) before and after curcumin (10 μ mol/L) treatment were examined by Western blotting. Real-time PCR and methylation specific PCR (MSP) were applied to analyze the expression and methylation level of ADAMTS18 gene before and after curcumin treatment (10 μ mol/L). Curcumin significantly inhibited the viability of A498 and 786-O cell lines in a dose- and time-dependent manner (P |
| Databáze: | OpenAIRE |
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