The 135 kbp mitochondrial genome of Agaricus bisporus is the largest known eukaryotic reservoir of group I introns and plasmid-related sequences
| Autor: | Jianping Xu, Cyril Férandon, Gérard Barroso |
|---|---|
| Přispěvatelé: | Unité de recherche Mycologie et Sécurité des Aliments (MycSA), Institut National de la Recherche Agronomique (INRA), Department of Biology, McMaster University |
| Rok vydání: | 2013 |
| Předmět: |
Mitochondrial DNA
Inverted repeat [SDV]Life Sciences [q-bio] Molecular Sequence Data Biology DNA Mitochondrial Microbiology Genome 03 medical and health sciences group I intron RNA Transfer Sequence Homology Nucleic Acid linear invertron-like plasmid Genetics Group I catalytic intron DNA Fungal Gene 030304 developmental biology 0303 health sciences inverted repeat 030306 microbiology basidiomycota Inverted Repeat Sequences Intron Sequence Analysis DNA Group II intron Introns mitochondria Genome Mitochondrial agaricus Mobile genetic elements Plasmids |
| Zdroj: | Fungal Genetics and Biology Fungal Genetics and Biology, Elsevier, 2013, 55, pp.85-91. ⟨10.1016/j.fgb.2013.01.009⟩ |
| ISSN: | 1087-1845 1096-0937 |
| DOI: | 10.1016/j.fgb.2013.01.009 |
| Popis: | International audience; At 135,005nt, the mitochondrial genome in Agaricus bisporus represents the largest fungal mitochondrial genome sequenced to date. Its large size is mainly due to the presence of mobile genetic elements, including a total of 43 group I introns, three group II introns, and five DNA fragments that show sequence similarity to linear invertron-like plasmids. The introns are distributed in eight of the 15 protein coding genes. These introns contain a total of 61,092nt (45.3% of the whole mitochondrial genome) and include representatives of most of the group I introns so far found in mitochondrial genomes of Basidiomycota. The plasmid-like sequences include 6730nt total representing 5.0% of the genome. These sequences showed high-level similarities to two different mitochondrial plasmids reported for basidiomycete mushrooms: the autonomously replicating pEM in Agaricus bitorquis and the integrated linear plasmid sequences in Agrocybe aegerita and Moniliophthora perniciosa. Moreover, the plasmid-related sequences are located within or adjacent to two large (4559nt) inverted repeats containing also two sets of mitochondrial tRNA genes. Our analyses are consistent with the hypothesis that horizontal DNA transfer has played a significant role in the evolution of the A. bisporus mitochondrial genome. |
| Databáze: | OpenAIRE |
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