Dose-responsive gene expression changes in juvenile and adult mummichogs (Fundulus heteroclitus) after arsenic exposure
| Autor: | William S. Baldwin, Jianjun Hu, Lisa J. Bain, Kristen M. Gaworecki, Horacio O. Gonzalez, Jorge L. Gardea-Torresdey, Jonathan A. Roling |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Sodium arsenite Apolipoprotein B Gene Expression Physiology chemistry.chemical_element 010501 environmental sciences Aquatic Science Oceanography 01 natural sciences Article Arsenic 03 medical and health sciences chemistry.chemical_compound Sex Factors Fundulidae Gene expression Animals Juvenile 030304 developmental biology 0105 earth and related environmental sciences Genetics 0303 health sciences Dose-Response Relationship Drug biology Aquatic animal General Medicine biology.organism_classification Pollution Fundulus Liver chemistry biology.protein Body Burden Female Growth and Development Water Pollutants Chemical |
| Zdroj: | Marine Environmental Research. 70:133-141 |
| ISSN: | 0141-1136 |
| DOI: | 10.1016/j.marenvres.2010.04.003 |
| Popis: | The present study investigated arsenic's effects on mummichogs (Fundulus heteroclitus), while also examining what role that gender or exposure age might play. Adult male and female mummichogs were exposed to 172 ppb, 575 ppb, or 1720 ppb arsenic as sodium arsenite for 10 days immediately prior to spawning. No differences were noted in the number or viability of eggs between the groups, but there was a significant increase in deformities in 1720 ppb arsenic exposure group. Total RNA from adult livers or 6-week old juveniles was used to probe custom macroarrays for changes in gene expression. In females, 3% of the genes were commonly differentially expressed in the 172 and 575 ppb exposure groups compared to controls. In the males, between 1.1 and 3% of the differentially expressed genes were in common between the exposure groups. Several genes, including apolipoprotein and serum amyloid precursor were commonly expressed in either a dose-responsive manner or were dose-specific, but consistent across genders. These patterns of regulation were confirmed by QPCR. These findings will provide us with a better understanding of the effects of dose, gender, and exposure age on the response to arsenic. |
| Databáze: | OpenAIRE |
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