Differential proteomics of Aedes albopictus salivary gland, midgut and C6/36 cell induced by dengue virus infection
| Autor: | Ai He, Zhuoya Li, Dongjing Zhang, Xiaoying Zheng, Xiansheng Wu, Ximei Zhan, Meichun Zhang, Yu Wu, Ming Gan |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Aedes albopictus
Proteome viruses Cell Dengue virus medicine.disease_cause Real-Time Polymerase Chain Reaction Virus Mass Spectrometry Salivary Glands Cell Line Aedes Virology medicine Animals Electrophoresis Gel Two-Dimensional RNA Messenger Differential proteomics Messenger RNA biology Salivary gland Gene Expression Profiling Midgut Dengue Virus biology.organism_classification Molecular biology Gastrointestinal Tract medicine.anatomical_structure Host-Pathogen Interactions |
| Zdroj: | Virology. 444(1-2) |
| ISSN: | 1096-0341 |
| Popis: | The interaction between dengue virus (DENV) and vector mosquitoes are still poorly understood at present. In this study, 2-D DIGE combined with MS was used to analyze the differential proteomes of Aedes albopictus salivary gland, midgut and C6/36 cells induced by DENV-2. Our results indicated that the virus infection regulated several functional classes of proteins. Among them, 26 were successfully analyzed by real-time RT-PCR. The mRNA levels of 15 were the highest in salivary gland, 2 in midgut and none in C6/36 cells, however, 18 were the least in fat body compared to other organs. Interestingly, the changes of differential proteins mRNA were the most obvious in fat body post-infection. Chaperone, cytoskeleton and energy metabolism enzyme were the most down- or up- regulated proteins after DENV-2 infection. The abundant expression of these proteins in salivary gland may relate to its high susceptibility. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect