P-cadherin-induced decorin secretion is required for collagen fiber alignment and directional collective cell migration
| Autor: | Cédric Plutoni, Franck Comunale, Evgeny V. Denisov, Stéphane Bodin, Elsa Bazellières, Maïlys Le Borgne-Rochet, L. A. Tashireva, Ivan Bièche, Cécile Gauthier-Rouvière, Martial Séveno, Lucie Angevin, Vladimir M. Perelmuter, Laura Ordas, Sophie Vacher |
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| Přispěvatelé: | Centre de recherche en Biologie Cellulaire (CRBM), Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1), Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS), Laboratory of Molecular Oncology and Immunology - Cancer Institute, National Research Tomsk State University, Department of General and Molecular Pathology, Genetique Moleculaire des Cancers d'Origine Epitheliale, Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Curie [Paris], Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), BioCampus Montpellier (BCM), Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie du Développement de Marseille-Luminy (IBDML), Université de la Méditerranée - Aix-Marseille 2-Centre National de la Recherche Scientifique (CNRS), Centre de recherche en Biologie cellulaire de Montpellier (CRBM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), BioCampus (BCM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU)-Collège de France (CdF)-Centre National de la Recherche Scientifique (CNRS), Centre de recherches de biochimie macromoléculaire (CRBM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-IFR122-Centre National de la Recherche Scientifique (CNRS), Institut Curie-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 1 (UM1)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Decorin
[SDV]Life Sciences [q-bio] Cell Morphogenesis CDC42 [SDV.BC]Life Sciences [q-bio]/Cellular Biology CDH1 Extracellular matrix 03 medical and health sciences 0302 clinical medicine Cell Movement medicine Cell Adhesion Humans cdc42 GTP-Binding Protein ComputingMilieux_MISCELLANEOUS 030304 developmental biology Mechanical Phenomena 0303 health sciences biology Cell migration Cell Biology Cadherins Cell biology Extracellular Matrix medicine.anatomical_structure Proteoglycan biology.protein Collagen 030217 neurology & neurosurgery |
| Zdroj: | Journal of Cell Science Journal of Cell Science, Company of Biologists, 2019, pp.jcs.233189. ⟨10.1242/jcs.233189⟩ Journal of Cell Science, Company of Biologists, 2019, 132 (21), pp.jcs233189. ⟨10.1242/jcs.233189⟩ Journal of Cell Science, 2019, 132 (21), pp.jcs233189. ⟨10.1242/jcs.233189⟩ Journal of Cell Science, 2019, pp.jcs.233189. ⟨10.1242/jcs.233189⟩ |
| ISSN: | 0021-9533 1477-9137 |
| DOI: | 10.1242/jcs.233189⟩ |
| Popis: | Directional collective cell migration (DCCM) is crucial for morphogenesis and cancer metastasis. P-cadherin (also known as CDH3), which is a cell–cell adhesion protein expressed in carcinoma and aggressive sarcoma cells and associated with poor prognosis, is a major DCCM regulator. However, it is unclear how P-cadherin-mediated mechanical coupling between migrating cells influences force transmission to the extracellular matrix (ECM). Here, we found that decorin, a small proteoglycan that binds to and organizes collagen fibers, is specifically expressed and secreted upon P-cadherin, but not E- and R-cadherin (also known as CDH1 and CDH4, respectively) expression. Through cell biological and biophysical approaches, we demonstrated that decorin is required for P-cadherin-mediated DCCM and collagen fiber orientation in the migration direction in 2D and 3D matrices. Moreover, P-cadherin, through decorin-mediated collagen fiber reorientation, promotes the activation of β1 integrin and of the β-Pix (ARHGEF7)/CDC42 axis, which increases traction forces, allowing DCCM. Our results identify a novel P-cadherin-mediated mechanism to promote DCCM through ECM remodeling and ECM-guided cell migration. |
| Databáze: | OpenAIRE |
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