Gas plasma-spurred wound healing is accompanied by regulation of focal adhesion, matrix remodeling, and tissue oxygenation
| Autor: | Thomas von Woedtke, Anke Schmidt, Grit Liebelt, Felix Nießner, Sander Bekeschus |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Hyperspectral imaging Clinical Biochemistry Biochemistry Focal adhesion Extracellular matrix 03 medical and health sciences Mice 0302 clinical medicine Cell Movement Cell Adhesion Animals Plasma medicine Reactive oxygen and nitrogen species Cell adhesion Myofibroblasts lcsh:QH301-705.5 Paxillin lcsh:R5-920 Focal Adhesions Wound Healing biology integumentary system Chemistry Organic Chemistry Vinculin Fibroblasts Dermal fibroblasts Cell biology Fibronectin 030104 developmental biology lcsh:Biology (General) biology.protein Wound healing lcsh:Medicine (General) 030217 neurology & neurosurgery Research Paper |
| Zdroj: | Redox Biology Redox Biology, Vol 38, Iss, Pp 101809-(2021) |
| ISSN: | 2213-2317 |
| Popis: | In response to injury, efficient migration of skin cells to rapidly close the wound and restore barrier function requires a range of coordinated processes in cell spreading and migration. Gas plasma technology produces therapeutic reactive species that promote skin regeneration by driving proliferation and angiogenesis. However, the underlying molecular mechanisms regulating gas plasma-aided cell adhesion and matrix remodeling essential for wound closure remain elusive. Here, we combined in vitro analyses in primary dermal fibroblasts isolated from murine skin with in vivo studies in a murine wound model to demonstrate that gas plasma treatment changed phosphorylation of signaling molecules such as focal adhesion kinase and paxillin α in adhesion-associated complexes. In addition to cell spreading and migration, gas plasma exposure affected cell surface adhesion receptors (e.g., integrinα5β1, syndecan 4), structural proteins (e.g., vinculin, talin, actin), and transcription of genes associated with differentiation markers of fibroblasts-to-myofibroblasts and epithelial-to-mesenchymal transition, cellular protrusions, fibronectin fibrillogenesis, matrix metabolism, and matrix metalloproteinase activity. Finally, we documented that gas plasma exposure increased tissue oxygenation and skin perfusion during ROS-driven wound healing. Altogether, these results provide critical insights into the molecular machinery of gas plasma-assisted wound healing mechanisms. Graphical abstract Other constituents of focal adhesions participate in the structural link between membrane receptors and the actin cytoskeleton [1]. A wound healing phase-dependent action of intracellular proteases (MMPs) senses extracellular signals after gas plasma treatment by modifying the activation state of structural proteins (e.g., vinculin and talin), which are associated with actin and integrins.Image 1 |
| Databáze: | OpenAIRE |
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