Improvement of electrophoresis performance by spectral analysis
| Autor: | René Kizek, Layal Abo Khayal, Ivo Provaznik |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Chromatography
Nucleic acid quantitation Chemistry Difference gel electrophoresis Analytical chemistry Laser Applied Microbiology and Biotechnology Horizontal electrophoresis laser spectral analysis agarose gel law.invention Wavelength Electrophoresis law Agarose gel electrophoresis Genetics Pulsed-field gel electrophoresis Absorption (electromagnetic radiation) Agronomy and Crop Science Molecular Biology Biotechnology |
| Zdroj: | African Journal of Biotechnology; Vol 11, No 51 (2012); 11329-11332 |
| ISSN: | 1684-5315 |
| Popis: | This paper describes a new design of standard agarose gel electrophoresis procedure for nucleic acids analysis. The electrophoresis was improved by using the real-time spectral analysis of the samples to increase its performance. A laser beam illuminated the analysed sample at wavelength with the highest absorption of gel components. Thus, DNA band is detected well before it may exits a gel plate. A modified horizontal electrophoresis device was designed, developed and tested.Keywords: Horizontal electrophoresis, laser, spectral analysis, agarose gel |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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