miRNA profiling during antigen-dependent T cell activation: A role for miR-132-3p
| Autor: | Marcos Fernández-Alfara, Fátima Sánchez-Cabo, Ana Rodríguez-Galán, Marta Ramírez-Huesca, Francisco Sánchez-Madrid, Cristina Gutiérrez-Vázquez, Dannys Jorge Martinez-Herrera, Alberto Pascual-Montano, María Mittelbrunn |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Instituto de Salud Carlos III, Fundación ProCNIC, UAM. Departamento de Medicina, Instituto de Investigación del Hospital de La Princesa (IP) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
CD4-Positive T-Lymphocytes EXPRESSION Medicina PREDICTION Science T cell Biology PI3K Article 03 medical and health sciences miR-132 P85-ALPHA Immune system Antigen Downregulation and upregulation microRNA medicine Pik3r1 gene Animals Humans MiR-132-3p PI3K/AKT/mTOR pathway Cells Cultured Messenger RNA Multidisciplinary microRNAs (miRNAs) T cell activation MICRORNA DICER Molecular biology 3. Good health Tumor Necrosis Factor Receptor Superfamily Member 7 Up-Regulation Class Ia Phosphatidylinositol 3-Kinase Mice Inbred C57BL MicroRNAs DUPLEXES 030104 developmental biology medicine.anatomical_structure HEK293 Cells DIFFERENTIATION Medicine MESSENGER-RNA |
| Zdroj: | Repisalud Instituto de Salud Carlos III (ISCIII) Repositorio Institucional de la Consejería de Sanidad de la Comunidad de Madrid Consejería de Sanidad de la Comunidad de Madrid Biblos-e Archivo. Repositorio Institucional de la UAM instname Scientific Reports, Vol 7, Iss 1, Pp 1-8 (2017) Scientific Reports |
| DOI: | 10.1038/s41598-017-03689-7 |
| Popis: | microRNAs (miRNAs) are tightly regulated during T lymphocyte activation to enable the establishment of precise immune responses. Here, we analyzed the changes of the miRNA profiles of T cells in response to activation by cognate interaction with dendritic cells. We also studied mRNA targets common to miRNAs regulated in T cell activation. pik3r1 gene, which encodes the regulatory subunits of PI3K p50, p55 and p85, was identified as target of miRNAs upregulated after T cell activation. Using 3′UTR luciferase reporter-based and biochemical assays, we showed the inhibitory relationship between miR-132-3p upregulation and expression of the pik3r1 gene. Our results indicate that specific miRNAs whose expression is modulated during T cell activation might regulate PI3K signaling in T cells This work was supported by grants SAF2014-55579R from Ministerio de Economía y Competitividad-Spain, ERC-2011-AdG 294340-GENTRIS, CIBER CARDIOVASCULAR (FEDER and Instituto de Salud Carlos III), PIE-13-00041 and INDISNET S2011-BMD-2332 (F. S. M.). The Centro Nacional de Investigaciones Cardiovasculares (CNIC, Spain) is supported by the Ministerio de Economía y Competitividad-Spain and the Pro-CNIC Foundation |
| Databáze: | OpenAIRE |
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