Pasteurella multocida enters polarized epithelial cells by interacting with host F-actin
| Autor: | Fern Tablin, Naomi J. Walker, Lori M. Hansen, Mireille J. Rabier, Nancy K. Tyler, Dwight C. Hirsh |
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| Rok vydání: | 1997 |
| Předmět: |
Pasteurella multocida
macromolecular substances Biology Kidney Immunofluorescence Microbiology Epithelium Cell Line law.invention chemistry.chemical_compound Dogs Confocal microscopy law medicine Animals Cytoskeleton Actin Cytochalasin D Microscopy Confocal Microvilli General Veterinary medicine.diagnostic_test General Medicine biology.organism_classification Actin cytoskeleton Antibodies Bacterial Actins Cell biology Actin Cytoskeleton Microscopy Electron chemistry Cell culture |
| Zdroj: | Veterinary Microbiology. 54:343-355 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/s0378-1135(96)01255-2 |
| Popis: | We investigated the interaction of an avian strain of Pasteurella multocida with the cytoskeleton of MDCK cells, which formed a polarized epithelium when grown on type I collagen coated filters. Bacteria were incubated with MDCK cells for 30 min. 2, 4 and 6 hours and their location and association with the cell cytoskeleton determined by double-label immunofluorescence confocal microscopy. Cells were stained with a polyclonal antiserum to the outer-membrane proteins of P. multocida and with rhodamine phalloidin which specifically binds filamentous (F) actin. Confocal microscopy revealed that bacteria entered the cells by 30 min, and that by 6 hours there was a marked alteration in the actin cytoskeleton in which long filaments were reorganized to discrete foci of short actin filaments, within which were one or more bacteria. Electron microscopy demonstrated that by 2 hours, each bacterium was associated with many short 5-6 nm filaments. Treatment of MDCK cells with cytochalasin D for either 30 minutes or 24 hours prior to infection disrupted the actin cytoskeleton and inhibited entry of P. multocida. |
| Databáze: | OpenAIRE |
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