TurnerFertility trial
| Autor: | M.j. Schleedoorn, Ina Beerendonk, Ron J. T. van Golde, Janiëlle A E M van Alfen-van der Velden, Didi D.M. Braat, Kathrin Fleischer, Ron Peek |
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| Přispěvatelé: | Obstetrie & Gynaecologie, MUMC+: MA Medische Staf Obstetrie Gynaecologie (9), RS: GROW - R4 - Reproductive and Perinatal Medicine |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Time Factors
Turner Syndrome ADOLESCENT ovarian reserve Cohort Studies 0302 clinical medicine Obstetrics and Gynaecology Turner syndrome YOUNG-ADULTS Protocol Ovarian tissue cryopreservation 030212 general & internal medicine Fertility preservation NETWORK Child OUTCOMES TRANSPLANTATIONS Obstetrics Vascular damage Radboud Institute for Molecular Life Sciences [Radboudumc 16] WOMEN General Medicine CANCER Women's cancers Radboud Institute for Health Sciences [Radboudumc 17] Observational Studies as Topic Treatment Outcome PREGNANCY Research Design Child Preschool Female CLINICAL-PRACTICE GUIDELINES Cohort study medicine.medical_specialty Ovarian Cortex fertility preservation live birth Healthcare improvement science Radboud Institute for Health Sciences [Radboudumc 18] 03 medical and health sciences medicine Humans Ovarian reserve Cryopreservation Pregnancy business.industry Ovary medicine.disease ONCOLOGY Transplantation business 030217 neurology & neurosurgery Follow-Up Studies |
| Zdroj: | BMJ Open, 9, 12, pp. e030855 BMJ Open BMJ Open, 9, e030855 BMJ Open, 9(12):030855, 1-7. BMJ Publishing Group |
| ISSN: | 2044-6055 |
| Popis: | ObjectiveTo investigate the occurrence of live birth in women with Turner syndrome (TS) after ovarian tissue cryopreservation in childhood followed by auto transplantation in adulthood and to find reliable prognostic markers for estimating the ovarian reserve in girls with TS in the future.SettingAn observational cohort study with long-term follow-up in a tertiary fertility clinic in the Netherlands. Patients recruitment between January 2018 and December 2021.Participants100 females aged 2 through 18 years with classical Turner (ie, 45,X0) or Turner variants (ie, 45,X mosaicism or structural anomalies). Girls with Y chromosomal content, minor X deletions with marginal impact on fertility, active HIV, hepatitis B or hepatitis C infection, and/or an absolute contra indication for surgery, anaesthesia or future pregnancy will be excluded.InterventionsOvarian cortical tissue will be harvested by performing a unilateral oophorectomy via laparoscopic approach. Ovarian cortex fragments will be prepared and cryopreserved. One fragment per patient will be used to determine follicular density by conventional histology, and to perform fluorescence in situ hybridisation analysis of ovarian cells. Routine chromosome analysis will be performed on both lymphocytes and buccal cells. A blood sample will be taken for hormonal analysis and all subjects will undergo a transabdominal ultrasound to determine the uterine and ovarian size. Patient characteristics, pregnancy rates and pregnancy outcomes will be collected from the patient’s medical record.Ethics and disseminationThe study protocol has been approved by the Central Committee on Research Involving Human Subjects in November 2017 (CCMO NL57738.000.16).Trial registration numberNCT03381300. |
| Databáze: | OpenAIRE |
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