Cellular and humoral adjuvant activity of lectins isolated from Korean mistletoe (Viscum album colaratum)
| Autor: | Tae Bong Kang, Taek Joon Yoon, Sung Hoon Kim, Yung Choon Yoo, Jong Bae Kim, Kabsu Kim, Ichiro Azuma, Erk Her |
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| Rok vydání: | 2001 |
| Předmět: |
Cellular immunity
T-Lymphocytes Immunology chemical and pharmacologic phenomena complex mixtures Immunoadjuvant Mice Immune system Adjuvants Immunologic Lectins Viscum album Immunology and Allergy Animals Hypersensitivity Delayed Plant Proteins Toxins Biological Pharmacology Immunity Cellular Mice Inbred BALB C Plants Medicinal biology Antibody titer hemic and immune systems biology.organism_classification biological factors Mistletoe Immunoglobulin Isotypes Ribosome Inactivating Proteins Type 2 Delayed hypersensitivity Humoral immunity Antibody Formation Hemocyanins biology.protein Cytokines Plant Preparations Antibody Plant Lectins therapeutics |
| Zdroj: | International immunopharmacology. 1(5) |
| ISSN: | 1567-5769 |
| Popis: | The adjuvant effect of lectins (KML-C) isolated from Korean mistletoe (Viscum album coloratum) on induction of humoral and cellular immune responses against keyhole limpet hemocyanine (KLH) was examined. When mice were immunized subcutaneously (s.c.) with KLH (20 micrograms/mouse) admixed with or without 50 ng/mouse of KML-C (KLH + KML-C), mice immunized with KLH + KML-C showed significantly higher antibody titers against KLH than those immunized with KLH alone, showing the highest titer 5 weeks after immunization. Furthermore, boost immunization with KLH + KML-C at 2-week interval elicited much higher activity than single immunization to enhance antibody responses against KLH. The assay for determining isotypes of antibodies revealed that KML-C augmented KLH-specific antibody titers of IgG1, IgG2a and IgG2b. The culture supernatants obtained from the splenocytes of mice treated with KLH + KML-C also showed a higher level of both KLH-specific Th-1 (IL-2 and IFN-gamma) and Th-2 type cytokine (IL-4). In an in vitro analysis of T lymphocyte proliferation to KLH on week 4, the splenocytes of mice treated with KLH + KML-C showed a significantly higher proliferating activity than those treated with KLH alone. In addition, mice immunized twice with KLH + KML-C and followed by intrafootpad (i.f.) injection of KLH (50 micrograms/site) 14 weeks after the primary immunization induced a higher delayed-type hypersensitivity (DTH) reaction than mice treated with KLH alone. These results suggest that KML-C is a potent immunoadjuvant to enhance cellular and humoral immune responses. |
| Databáze: | OpenAIRE |
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