W361R mutation in GaaR, the regulator of D-galacturonic acid-responsive genes, leads to constitutive production of pectinases in Aspergillus niger
| Autor: | Simon B. Otto, Thi T. M. Pham, Adrian Tsang, Ebru Alazi, Mark Arentshorst, Arthur F. J. Ram, Jing Niu |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
food.ingredient Transcription Genetic Pectin 030106 microbiology Mutation Missense lcsh:QR1-502 Repressor medicine.disease_cause Microbiology lcsh:Microbiology 03 medical and health sciences chemistry.chemical_compound food Gene Expression Regulation Fungal medicine constitutively active transcription factor Pectinase Gene Mutation biology Chemistry missense mutation Aspergillus niger Promoter Original Articles transcription factor localization biology.organism_classification pectinase Polygalacturonase 030104 developmental biology Biochemistry Original Article Mutant Proteins CRISPR‐Cas9 D-Galacturonic acid Transcription Factors |
| Zdroj: | MicrobiologyOpen, 8(5), e00732 MicrobiologyOpen, Vol 8, Iss 5, Pp n/a-n/a (2019) MicrobiologyOpen |
| Popis: | Polysaccharides present in plant biomass, such as pectin, are the main carbon source for filamentous fungi. Aspergillus niger naturally secretes pectinases to degrade pectin and utilize the released monomers, mainly D‐galacturonic acid. The transcriptional activator GaaR, the repressor of D‐galacturonic acid utilization GaaX, and the physiological inducer 2‐keto‐3‐deoxy‐L‐galactonate play important roles in the transcriptional regulation of D‐galacturonic acid‐responsive genes, which include the genes encoding pectinases. In this study, we described the mutations found in gaaX and gaaR that enabled constitutive (i.e., inducer‐independent) expression of pectinases by A. niger. Using promoter‐reporter strains (PpgaX‐amdS) and polygalacturonic acid plate assays, we showed that W361R mutation in GaaR results in constitutive production of pectinases. Analysis of subcellular localization of C‐terminally eGFP‐tagged GaaR/GaaRW 361R revealed important differences in nuclear accumulation of N‐ versus C‐terminally eGFP‐tagged GaaR. |
| Databáze: | OpenAIRE |
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