The radioimmunoassay of buprenorphine
| Autor: | M. J. Rance, J. G. Lloyd-Jones, G. J. Dockray, R A Moore, I. R. Flockhart, M. R. D. Bennett, A. J. Bartlett |
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| Rok vydání: | 1980 |
| Předmět: |
Male
Radioimmunoassay Pharmacology chemistry.chemical_compound Dogs Antibody Specificity Blood plasma medicine Animals Humans Pharmacology (medical) Norbuprenorphine Bovine serum albumin Antiserum biology Chemistry General Medicine Plasma levels Buprenorphine Morphinans biology.protein Rabbits medicine.drug Conjugate |
| Zdroj: | European Journal of Clinical Pharmacology. 18:339-345 |
| ISSN: | 1432-1041 0031-6970 |
| DOI: | 10.1007/bf00561392 |
| Popis: | Antisera to buprenorphine were obtained in rabbits immunised with 3-0-carboxymethylbuprenorphine and N-hemisuccinyl-norbuprenorphine conjugated to bovine serum albumin. Using the latter antiserum and tritium labelled buprenorphine a radioimmunoassay have good accuracy and precision was developed for concentrations as low as 50 picograms in 1 ml of plasma. The N-hemisuccinyl antiserum crossreacted with norbuprenorphine, and the 3-0-glucuronide conjugate with the 3-0-carboxymethyl antiserum. Cross-reactivity of both antisera to other pharmacologically related compounds was negligible. The assay was employed to determine plasma buprenorphine concentration following its parenteral administration to dog and man. |
| Databáze: | OpenAIRE |
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