Phytochemical potential of Daphne gnidium in inhibiting growth of melanoma cells and enhancing melanogenesis of B16‐F0 melanoma
| Autor: | Alain Simon, Aline Pinon, Leila Chekir-Ghedira, Kamel Ghedira, Fadwa Chaabane |
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| Rok vydání: | 2012 |
| Předmět: |
Cell Survival
Tyrosinase Clinical Biochemistry Flavonoid Melanoma Experimental Ethyl acetate Acetates Biochemistry Melanin Mice chemistry.chemical_compound Cell Line Tumor Animals Humans Viability assay Cell Proliferation chemistry.chemical_classification biology Plant Extracts Chemistry Cell Biology General Medicine Cell cycle Flow Cytometry biology.organism_classification Plant Leaves Daphne gnidium Polyphenol Daphne |
| Zdroj: | Cell Biochemistry and Function. 31:460-467 |
| ISSN: | 1099-0844 0263-6484 |
| Popis: | In this study, we have investigated inhibitory capacity of ethyl acetate, total oligomer flavonoid (TOF), aqueous extracts and beta amyrin acetate, a triterpene isolated from ethyl acetate extract obtained from leaves of Daphne gnidium, on mouse melanoma (B16-F0 and B16-F10 cells) proliferation. Influence of these products on percentage cell distribution in cycle phases and melanogenesis was also studied. Cell viability was determined using the 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and flow cytometry was used to analyse effects of tested compounds on progression through the cell cycle. In addition, amounts of melanin and tyrosinase were measured spectrophotometrically at 475 nm. Ethyl acetate, TOF and aqueous extracts exhibited significant anti-proliferative activity after incubation with the two types of tumour skin cells B16-F0 and B16-F10. Furthermore, cell cycle analysis revealed that cells treated with ethyl acetate and TOF extracts were arrested predominantly in G2-M phase. Ethyl acetate extract has also the ability to enhance melanogenesis and tyrosinase activity of B16-F0 melanoma cells. Copyright © 2012 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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