Rapid approach to identify the presence of Arabica and Robusta species in coffee using 1H NMR spectroscopy
| Autor: | Thomas Kuballa, Bernd Diehl, Ole Winkelmann, Yulia B. Monakhova, Freddy Thomas, Dirk W. Lachenmeier, Maren Ilse, Winfried Ruge |
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| Rok vydání: | 2015 |
| Předmět: |
Detection limit
Principal Component Analysis 1h nmr spectroscopy Chemistry Proton Magnetic Resonance Spectroscopy Analytical chemistry Water Coffea General Medicine Nuclear magnetic resonance spectroscopy Analytical Chemistry chemistry.chemical_compound Seeds Principal component analysis Diterpenes Food Analysis Food Science Kahweol |
| Zdroj: | Food Chemistry. 182:178-184 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2015.02.132 |
| Popis: | NMR spectroscopy was used to verify the presence of Arabica and Robusta species in coffee. Lipophilic extracts of authentic roasted and green coffees showed the presence of established markers for Robusta (16-O-methylcafestol (16-OMC)) and for Arabica (kahweol). The integration of the 16-OMC signal (δ 3.165 ppm) was used to estimate the amount of Robusta in coffee blends with an approximate limit of detection of 1–3%. The method was successfully applied for the analysis of 77 commercial coffee samples (coffee pods, coffee capsules, and coffee beans). Furthermore, principal component analysis (PCA) was applied to the spectra of lipophilic and aqueous extracts of 20 monovarietal authentic samples. Clusters of the two species were observed. NMR spectroscopy can be used as a rapid prescreening tool to discriminate Arabica and Robusta coffee species before the confirmation applying the official method. |
| Databáze: | OpenAIRE |
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