Polyphenols-Loaded Sericin Self-Assembling Nanoparticles: A Slow-Release for Regeneration by Tissue-Resident Mesenchymal Stem/Stromal Cells
| Autor: | Elia Bari, Milena Sorrenti, Maria Luisa Torre, Silvio Faragò, L. Segale, Marzio Sorlini, Carla Renata Arciola, Giulia Orlandi, Laura Catenacci, Sara Perteghella |
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| Přispěvatelé: | Orlandi, Giulia, Bari, Elia, Catenacci, Laura, Sorrenti, Milena, Segale, Lorena, Faragò, Silvio, Sorlini, Marzio, Arciola, Carla Renata, Torre, Maria Luisa, Perteghella, Sara |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
epigallocatechin gallate
Antioxidant Stromal cell medicine.medical_treatment lcsh:RS1-441 Pharmaceutical Science Silk-sericin nanoparticles 02 engineering and technology tissue regeneration Epigallocatechin gallate Sericin Article quercetin lcsh:Pharmacy and materia medica 03 medical and health sciences chemistry.chemical_compound medicine Proanthocyanidins mesenchymal stem/stromal cells proanthocyanidin 030304 developmental biology 0303 health sciences Regeneration (biology) Mesenchymal stem cell 021001 nanoscience & nanotechnology Controlled release In vitro silk-sericin nanoparticle Cell biology chemistry 0210 nano-technology |
| Zdroj: | Pharmaceutics, Vol 12, Iss 381, p 381 (2020) Pharmaceutics Volume 12 Issue 4 |
| Popis: | Mesenchymal stem/stromal cells (MSCs) are a therapeutic target to promote tissue regeneration, mainly when oxidative stress-mediated damage is involved in disease pathogenesis. Here, slow-release silk sericin nanoparticles (SNPs) loaded with natural antioxidant polyphenols were developed to sustain regeneration by tissue-resident MSCs. SNPs were prepared by exploiting a self-assembly method with poloxamer and were loaded with proanthocyanidins (P), quercetin (Q) or epigallocatechin gallate (E). SNPs, with a diameter less than 150 nm, were able to encapsulate both hydrophilic (P and E) and hydrophobic (Q) drugs. A slow and controlled release was obtained from SNPs for all the actives in PBS, while in EtOH, Q and E showed a burst release but P did not. Kinetic models revealed lower diffusion of P than other biomolecules, probably due to the higher steric hindrance of P. The in vitro anti-oxidant, anti-elastase and anti-tyrosinase properties of SNPs were assessed: loading the P and E into SNPs preserved the in vitro biological activities whereas for Q, the anti-elastase activity was strongly improved. Moreover, all formulations promoted MSC metabolic activity over 72 h. Finally, SNPs exhibited a strong ability to protect MSCs from oxidative stress, which supports their potential use for regenerative purposes mediated by tissue-resident MSCs. |
| Databáze: | OpenAIRE |
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