Putative resistance gene markers associated with quantitative trait loci for fire blight resistance in Malus 'Robusta 5' accessions

Autor: Susan E. Gardiner, Angela M. Baldo, Nihal De Silva, Gennaro Fazio, Claudia Wiedow, Mickael Malnoy, Klaus Richter, Jean-Marc Celton, C. M. Carlisle, Herb S. Aldwinckle, Yizhen Wan, M.B. Horner, Carole L. Bassett, Vincent G. M. Bus, John L. Norelli, Andreas Peil, Deepa Bowatte
Přispěvatelé: Manawatu Mail Ctr, New Zealand Inst Plant & Food Res Ltd PFR Palmers, USDA, Appalachian Fruit Res Stn, Agricultural Research Service, Auckland Mail Ctr, PFR Mt Albert, USDA, Plant Genet Resources Unit, Inst Breeding Res Hort & Fruit Crops, JKI, Fdn E Mach, Ist Agr San Michele allAdige, PFR Hawkes Bay, Coll Hort, Yangling, NW A&F Univ, Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Inst Resistance Res & Stress, Dept Plant Pathol & Plant Microbe Biol, Cornell University, National Research Initiative of the USDA Cooperative State Research, Education and Extension Service [2005-35300-15462], New Zealand Foundation for Science, Technology and Research [C02X0406, C06X0810], Plant & Food Research, USDA-ARS : Agricultural Research Service, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Cornell University [New York]
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Zdroj: BMC Genetics
BMC Genetics, BioMed Central, 2012, 13, ⟨10.1186/1471-2156-13-25⟩
BMC Genetics, Vol 13, Iss 1, p 25 (2012)
BMC Genetics (13), . (2012)
ISSN: 1471-2156
Popis: Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; Background: Breeding of fire blight resistant scions and rootstocks is a goal of several international apple breeding programs, as options are limited for management of this destructive disease caused by the bacterial pathogen Erwinia amylovora. A broad, large-effect quantitative trait locus (QTL) for fire blight resistance has been reported on linkage group 3 of Malus 'Robusta 5'. In this study we identified markers derived from putative fire blight resistance genes associated with the QTL by integrating further genetic mapping studies with bioinformatics analysis of transcript profiling data and genome sequence databases. Results: When several defined E. amylovora strains were used to inoculate three progenies from international breeding programs, all with 'Robusta 5' as a common parent, two distinct QTLs were detected on linkage group 3, where only one had previously been mapped. In the New Zealand 'Malling 9' X 'Robusta 5' population inoculated with E. amylovora ICMP11176, the proximal QTL co-located with SNP markers derived from a leucine-rich repeat, receptor-like protein (MxdRLP1) and a closely linked class 3 peroxidase gene. While the QTL detected in the German 'Idared' X 'Robusta 5' population inoculated with E. amylovora strains Ea222_JKI or ICMP11176 was approximately 6 cM distal to this, directly below a SNP marker derived from a heat shock 90 family protein gene (HSP90). In the US 'Otawa3' X 'Robusta5' population inoculated with E. amylovora strains Ea273 or E2002a, the position of the LOD score peak on linkage group 3 was dependent upon the pathogen strains used for inoculation. One of the five MxdRLP1 alleles identified in fire blight resistant and susceptible cultivars was genetically associated with resistance and used to develop a high resolution melting PCR marker. A resistance QTL detected on linkage group 7 of the US population co-located with another HSP90 gene-family member and a WRKY transcription factor previously associated with fire blight resistance. However, this QTL was not observed in the New Zealand or German populations. Conclusions: The results suggest that the upper region of 'Robusta5' linkage group 3 contains multiple genes contributing to fire blight resistance and that their contributions to resistance can vary depending upon pathogen virulence and other factors. Mapping markers derived from putative fire blight resistance genes has proved a useful aid in defining these QTLs and developing markers for marker-assisted breeding of fire blight resistance.
Databáze: OpenAIRE
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