Analysis of ligand-dependent recruitment of coactivator peptides to RXRβ in a time-resolved fluorescence resonance energy transfer assay
| Autor: | Deborah K. Stafslien, Kevin L. Vedvik, Mary Szatkowski Ozers, Therese De Rosier |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Amino Acid Motifs
Retinoic acid Peroxisome proliferator-activated receptor Tretinoin Peptide Retinoid X receptor Ligands Biochemistry chemistry.chemical_compound Endocrinology Coactivator Fluorescence Resonance Energy Transfer Humans Liver X receptor Molecular Biology Adaptor Proteins Signal Transducing Retinoid X Receptor beta chemistry.chemical_classification Protein Structure Tertiary Kinetics Förster resonance energy transfer chemistry Nuclear receptor Biophysics Peptides hormones hormone substitutes and hormone antagonists Protein Binding Signal Transduction |
| Zdroj: | Molecular and Cellular Endocrinology. 264:82-89 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/j.mce.2006.10.016 |
| Popis: | Because RXR plays a significant role in nuclear receptor signaling as a common heterodimeric partner for TR, PPAR, RAR, VDR, LXR and others, the ability of RXRbeta ligand binding domain (LBD) to interact with coregulator peptides bearing LXXLL or other interaction motifs was investigated using time-resolved fluorescence resonance energy transfer (TR-FRET). The random phage display peptide D22 and peptides derived from PGC1alpha, SRC1-4, SRC2-3, PRIP/RAP250 and RIP140 yielded the highest TR-FRET signal with RXRbeta LBD in the presence of saturating 9-cis retinoic acid (9-cisRA). Several peptides including D22, PGC1alpha, SRC3-2, PRIP/RAP250 and SRC1-4 also formed a complex with RXRbeta LBD in the presence of all-trans retinoic acid (at-RA) and the fatty acids, phytanic acid (PA) and docosahexaenoic acid (DHA). Determination of the dose dependency (EC50) of these compounds to recruit D22 to RXRbeta LBD indicated that the rank order potency was 9-cisRAPAat-RADHA. The ligands 9-cisRA and at-RA yielded an overall higher fold-change in D22 recruitment to RXRbeta LBD suggesting that more RXRbeta LBD-D22 complex was formed in the presence of these ligands under the assay conditions tested. The statistical parameter Z' factor for 9-cisRA-induced recruitment of D22 to RXRbeta LBD was 0.6 after 2h incubation, indicating a robust methodology that could be applied to high throughput screening. These results demonstrate that RXRbeta occupied with the fatty acid ligands, DHA and PA, can recruit coactivator peptides in a ligand-dependent manner. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect