Accuracy and stability of saliva as a sample for reverse transcription PCR detection of SARS-CoV-2
| Autor: | Hiroki Kabata, Toshiki Shiraki, Shunsuke Uno, Yuko Sakai-Tagawa, Koichi Fukunaga, Shinnosuke Ikemura, Yuta Fujimori, Hirofumi Kamata, Kiyoko Iwatsuki-Horimoto, Yoshifumi Uwamino, Terumichi Nakagawa, Mika Nagata, Mitsuru Murata, Sho Uchida, Naoki Hasegawa, Wataru Aoki, Hiromitsu Yokota, Makoto Ishii, Yoshihiro Kawaoka |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Saliva Coronavirus disease 2019 (COVID-19) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Sample (material) Pathology and Forensic Medicine law.invention Specimen Handling 03 medical and health sciences 0302 clinical medicine law Internal medicine medicine Humans Pandemics Polymerase chain reaction business.industry Reverse Transcriptase Polymerase Chain Reaction SARS-CoV-2 COVID-19 Reproducibility of Results General Medicine University hospital Reverse transcription polymerase chain reaction 030104 developmental biology 030220 oncology & carcinogenesis RNA Viral Sample collection business |
| Zdroj: | Journal of Clinical Pathology |
| ISSN: | 1472-4146 |
| Popis: | COVID-19 prevalence has increased worldwide. Reverse transcription (RT)-PCR-based SARS-CoV-2 detection has majorly contributed to COVID-19 diagnosis. Although nasopharyngeal swab samples are commonly used for RT-PCR, infection risk is high among the healthcare personnel during sample collection. Saliva, which can be self-collected by patients even at home, has been proposed as a sample for RT-PCR-based SARS-CoV-2 detection, thus potentially reducing the infection risk among healthcare personnel.1 2 However, few studies have assessed the accuracy of RT-PCR analysis using multiple saliva samples. Furthermore, salivary ribonuclease is speculated to affect the analysis of stored samples.3 From 15 May to 16 July 2020, we obtained nasopharyngeal swabs and saliva samples simultaneously, from patients admitted to Keio University Hospital (Tokyo, Japan) for COVID-19 treatment and from the university staff presenting symptoms suggesting acute viral infections, including fever, upper or lower respiratory symptoms, or diarrhoea. Nasopharyngeal swab samples were collected by trained medical staff using a FLOQ SWAB and a BD UVT container (BD, Franklin Lakes, New Jersey, USA), and saliva samples were collected by patients themselves in sterile containers after 1 min of salivation. Real-time RT-PCR-based SARS-CoV-2 detection was simultaneously performed for both samples, using LightCycler96 (Roche, Basel, Switzerland) using the 2019 Novel Coronavirus Detection Kit (Shimadzu, Kyoto, Japan) in accordance with the manufacturer’s instructions using N1 and N2 primers and probes.4 Ct values of |
| Databáze: | OpenAIRE |
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