Phaseolus acutifolius Lectin Fractions Exhibit Apoptotic Effects on Colon Cancer: Preclinical Studies Using Dimethilhydrazine or Azoxi-Methane as Cancer Induction Agents

Autor: Alejandro Blanco-Labra, Josue Lopez-Martinez, Carmen Mejía, Roberto Augusto Ferriz-Martínez, María de Jesús Guerrero-Carrillo, Teresa García-Gasca, Alejandro Eduardo García-Pascalin, Ulisses Moreno-Celis, Ricardo Cervantes-Jiménez, Laura Elena Estrada-Martínez, Adriana Jheny Rodríguez-Méndez
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
Colorectal cancer
Pharmaceutical Science
Pharmacology
medicine.disease_cause
Phaseolus acutifolius
Analytical Chemistry
Rats
Sprague-Dawley
Mice
0302 clinical medicine
Drug Discovery
Tepary Bean
Phaseolus
Caspase 3
apoptosis
3T3 Cells
Biochemistry
colon cancer
Chemistry (miscellaneous)
030220 oncology & carcinogenesis
Colonic Neoplasms
Seeds
Molecular Medicine
Signal transduction
Plant Lectins
Signal Transduction
Cell Survival
Biology
Article
lcsh:QD241-441
Tepary bean
03 medical and health sciences
food
lcsh:Organic chemistry
medicine
Animals
Physical and Theoretical Chemistry
Protein kinase B
lectins
Cell Proliferation
Plant Extracts
Organic Chemistry
Lectin
medicine.disease
Antineoplastic Agents
Phytogenic
food.food
030104 developmental biology
Apoptosis
biology.protein
Carcinogenesis
Zdroj: Molecules; Volume 22; Issue 10; Pages: 1670
Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry
Molecules, Vol 22, Iss 10, p 1670 (2017)
ISSN: 1420-3049
DOI: 10.3390/molecules22101670
Popis: Phaseolus acutifolius (Tepary bean) lectins have been studied as cytotoxic molecules on colon cancer cells. The toxicological profile of a Tepary bean lectin fraction (TBLF) has shown low toxicity in experimental animals; exhibiting anti-nutritional effects such as a reduction in body weight gain and a decrease in food intake when using a dose of 50 mg/kg on alternate days for six weeks. Taking this information into account, the focus of this work was to evaluate the effect of the TBLF on colon cancer using 1,2-dimethylhydrazine (DMH) or azoxy-methane/dextran sodium sulfate (AOM/DSS) as colon cancer inductors. Rats were treated with DMH or AOM/DSS and then administered with TBFL (50 mg/kg) for six weeks. TBLF significantly decreased early tumorigenesis triggered by DMH by 70%, but without any evidence of an apoptotic effect. In an independent experiment, AOM/DSS was used to generate aberrant cryptic foci, which decreased by 50% after TBLF treatment. TBLF exhibited antiproliferative and proapoptotic effects related to a decrease of the signal transduction pathway protein Akt in its activated form and an increase of caspase 3 activity, but not to p53 activation. Further studies will deepen our knowledge of specific apoptosis pathways and cellular stress processes such as oxidative damage.
Databáze: OpenAIRE
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