A-to-I miR-378a-3p editing can prevent melanoma progression via regulation of PARVA expression
| Autor: | Cristina Ivan, A. Gordon Robertson, Victoria K. Xie, Gabriel Lopez-Berenstein, Guermarie Velazquez-Torres, Li Huang, Einav Shoshan, Anil K. Sood, Harrison Paret, George A. Calin, Menashe Bar-Eli, Sun Jin Kim, Steven J.M. Jones, Enrique Fuentes-Mattei, Cristian Rodriguez-Aguayo, Denise Brooks |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adenosine Skin Neoplasms Science General Physics and Astronomy Mice Nude Biology General Biochemistry Genetics and Molecular Biology Article Metastasis Epigenesis Genetic 03 medical and health sciences Mice Cell Line Tumor microRNA medicine Animals Humans Neoplasm Invasiveness Cancer epigenetics Neoplasm Metastasis lcsh:Science 3' Untranslated Regions Melanoma Cell Proliferation Regulation of gene expression Multidisciplinary Oncogene Three prime untranslated region Microfilament Proteins General Chemistry Oncogenes medicine.disease Inosine 3. Good health Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology RNA editing Cancer research Disease Progression lcsh:Q Female RNA Editing |
| Zdroj: | Nature Communications Nature Communications, Vol 9, Iss 1, Pp 1-7 (2018) |
| ISSN: | 2041-1723 |
| Popis: | Previously we have reported that metastatic melanoma cell lines and tumor specimens have reduced expression of ADAR1 and consequently are impaired in their ability to perform A-to-I microRNA (miRNA) editing. The effects of A-to-I miRNAs editing on melanoma growth and metastasis are yet to be determined. Here we report that miR-378a–3p is undergoing A-to-I editing only in the non-metastatic but not in metastatic melanoma cells. The function of the edited form is different from its wild-type counterpart. The edited form of miR-378a-3p preferentially binds to the 3′-UTR of the PARVA oncogene and inhibits its expression, thus preventing the progression of melanoma towards the malignant phenotype. Indeed, edited miR-378a-3p but not its WT form inhibits melanoma metastasis in vivo. These results further emphasize the role of RNA editing in melanoma progression. In melanoma, reduced ADAR1 impairs A-to-I microRNA editing. Here, the authors show that miR-378a-3p undergoes this editing in non-metastatic cells and the edited form of miR-378a-3p binds to the PARVA oncogene, inhibiting its expression and preventing melanoma progression and metastasis. |
| Databáze: | OpenAIRE |
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