The adherence-associated Fdp fasciclin I domain protein of the biohydrogen producer
| Autor: | E.-L. Jeong, Robert G. Moody, S.J. Broad, Mary K. Phillips-Jones |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Fasciclin domain
Mutant Protein domain Energy Engineering and Power Technology 02 engineering and technology Rhodobacter sphaeroides 010402 general chemistry 01 natural sciences Article chemistry.chemical_compound Binding site Regulation of gene expression Growth medium biology Prr two-component system Renewable Energy Sustainability and the Environment Green energy Wild type 021001 nanoscience & nanotechnology Condensed Matter Physics biology.organism_classification 0104 chemical sciences Cell biology Gene regulation Fuel Technology chemistry 0210 nano-technology Biohydrogen |
| Zdroj: | International Journal of Hydrogen Energy |
| ISSN: | 0360-3199 |
| Popis: | Expression of fdp, encoding a fasciclin I domain protein important for adherence in the hydrogen-producing bacterium Rhodobacter sphaeroides, was investigated under a range of conditions to gain insights into optimization of adherence for immobilization strategies suitable for H2 production. The fdp promoter was linked to a lacZ reporter and expressed in wild type and in PRRB and PRRA mutant strains of the Prr regulatory pathway. Expression was significantly negatively regulated by Prr under all conditions of aerobiosis tested including anaerobic conditions (required for H2 production), and aerobically regardless of growth phase, growth medium complexity or composition, carbon source, heat and cold shock and dark/light conditions. Negative fdp regulation by Prr was reflected in cellular levels of translated Fdp protein. Since Prr is required directly for nitrogenase expression, we propose optimization of Fdp-based adherence in R. sphaeroides for immobilized biohydrogen production by inactivation of the PrrA binding site(s) upstream of fdp. Highlights • Adherence and immobilization of hydrogen-producing microbes improves H2 yields. • fdp encodes an adherence factor in hydrogen-producer Rhodobacter sphaeroides. • Expression of fdp is negatively regulated by the global Prr regulatory pathway. • Negative regulation by Prr was demonstrated under all growth conditions tested. • Fdp-based adherence may be optimised by inactivating the PrrA binding site(s) of fdp. |
| Databáze: | OpenAIRE |
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