Grafting response to excess boron and expression analysis of genes coding boron transporters in tomato

Autor: Pietro Santamaria, Alberto Pardossi, Alessio Aprile, Erika Sabella, Eliana Nutricati, L. De Bellis, F. Di Gioia, Antonio Miceli
Přispěvatelé: Di Gioia, F., Aprile, A., Sabella, E., Santamaria, P., Pardossi, A., Miceli, A., De Bellis, L., Nutricati, E.
Rok vydání: 2017
Předmět:
0106 biological sciences
0301 basic medicine
vegetable grafting
boron transporter
Plant Science
01 natural sciences
Plant Roots
Solanum lycopersicum
Gene Expression Regulation
Plant
Solanum lycopersicum × Solanum habrochaite
Cultivar
Plant Proteins
biology
Ecology
food and beverages
Plant Protein
General Medicine
Grafting
SlNIP5
Shoot
Rootstock
Plant Leave
Plant Shoots
Evolution
chemistry.chemical_element
Plant Shoot
03 medical and health sciences
Behavior and Systematics
Botany
Solanum lycopersicum × Solanum habrochaites
Lycopersicon esculentum
Boron
Gene
Ecology
Evolution
Behavior and Systematics
SlBOR1
fungi
boron transporters
rootstock
Plant Leaves
Transporter
Plant Root
Plant
biology.organism_classification
Ecology
Evolution
Behavior and Systematic
030104 developmental biology
chemistry
Gene Expression Regulation
Solanum
010606 plant biology & botany
Zdroj: Plant biology (Stuttgart, Germany). 19(5)
ISSN: 1438-8677
Popis: Boron (B) is essential for plant growth, however its excess in soil and/or in irrigation water can severely compromise plant growth and yield. The goal of this work was to determine whether grafting onto 'Arnold', a commercial interspecific hybrid (Solanum lycopersicum × S. habrochaites) rootstock, which in a previous study was found to be tolerant to salt stress, could improve tomato (S. lycopersicum L. 'Ikram') tolerance to excess B, and whether this effect is associated with an exclusion mechanism. Non-grafted, self-grafted and grafted plants were hydroponically grown in a greenhouse with B concentration in the nutrient solution of 0.27 (control), 5, 10 and 15 mg·l-1 . A transcription analysis was carried out on SlNIP5 and SlBOR1 genes, which encode putative B transporters. Grafting 'Ikram' onto 'Arnold' rootstock reduced B concentration in leaf tissue of plants exposed to B concentrations of 10-15 mg·l-1 . At high B levels, SlNIP5 was down-regulated in all grafting combinations, while SlBOR1 was down-regulated only in the roots of plants grafted onto 'Arnold'. We conclude that grafting the susceptible tomato cultivar 'Ikram' onto the commercial rootstock 'Arnold' improved tolerance to excess B by reducing expression of genes encoding for B transporters at the root level, thus partially reducing the root uptake of B and its accumulation in the shoot.
Databáze: OpenAIRE
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