Screening of Lactobacillus spp. for the prevention of Pseudomonas aeruginosa pulmonary infections

Autor: Rozenn Le Berre, Youenn Alexandre, Gwenaelle Le Blay, Georges Barbier
Přispěvatelé: Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM), Université de Brest (UBO), Département de Médecine Interne et Pneumologie [Brest] (DMIP - Brest), Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Laboratory of Food Biotechnology, Institute of Food Science and Nutrition, Laboratoire de microbiologie des environnements extrêmophiles (LM2E), Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)
Rok vydání: 2014
Předmět:
Male
chloramphenicol
kanamycin
antibiotic resistance
[SDV]Life Sciences [q-bio]
vancomycin
medicine.disease_cause
biofilm
Elastolytic activity
RNA
Ribosomal
16S
Ampicillin
quinupristin
dalfopristin
mass spectrometry
Aged
80 and over
clinical article
milk
0303 health sciences
lung infection
raw milk
article
Lactobacillus vaginalis
food and beverages
mouth cavity
Middle Aged
Lactobacillus salivarius
Healthy Volunteers
Electrophoresis
Gel
Pulsed-Field
3. Good health
saliva substitute
Lactobacillus zeae
erythromycin
Pseudomonas aeruginosa
Lactobacillus fermentum
Female
Pseudomonas infection
Gentamicin
Lactobacillus casei
Research Article
medicine.drug
Adult
DNA
Bacterial
Microbiology (medical)
Molecular Sequence Data
Lactobacillus reuteri
Virulence
gene sequence
pulsed field gel electrophoresis
gentamicin
Biology
DNA
Ribosomal
Microbiology
Young Adult
03 medical and health sciences
Antibiotic resistance
Bacterial Proteins
Lactobacillus rhamnosus
Organic acids
Antibiosis
bacterium isolation
medicine
Animals
Humans
controlled study
human
Lactobacillus paracasei
Biofilm formation
Aged
tetracycline
030304 developmental biology
Mouth
nonhuman
030306 microbiology
Probiotics
bacterial virulence
nucleotide sequence
Sequence Analysis
DNA
clindamycin
bacterial strain
medicine.disease
biology.organism_classification
Lactobacillus
ampicillin
Lactobacillus plantarum
Zdroj: BMC Microbiology
BMC Microbiology, BioMed Central, 2014, 14, pp.107. ⟨10.1186/1471-2180-14-107⟩
Bmc Microbiology (1471-2180) (Biomed Central Ltd), 2014-04, Vol. 14, N. 107, P. 1-10
ISSN: 1471-2180
DOI: 10.1186/1471-2180-14-107
Popis: International audience; BACKGROUND: Pseudomonas aeruginosa is an opportunistic pathogen that significantly increases morbidity and mortality in nosocomial infections and cystic fibrosis patients. Its pathogenicity especially relies on the production of virulence factors or resistances to many antibiotics. Since multiplication of antibiotic resistance can lead to therapeutic impasses, it becomes necessary to develop new tools for fighting P. aeruginosa infections. The use of probiotics is one of the ways currently being explored. Probiotics are microorganisms that exert a positive effect on the host's health and some of them are known to possess antibacterial activities. Since most of their effects have been shown in the digestive tract, experimental data compatible with the respiratory environment are strongly needed. The main goal of this study was then to test the capacity of lactobacilli to inhibit major virulence factors (elastolytic activity and biofilm formation) associated with P. aeruginosa pathogenicity. RESULTS: Sixty-seven lactobacilli were isolated from the oral cavities of healthy volunteers. These isolates together with 20 lactobacilli isolated from raw milks, were tested for their capacity to decrease biofilm formation and activity of the elastase produced by P. aeruginosa PAO1. Ten isolates, particularly efficient, were accurately identified using a polyphasic approach (API 50 CHL, mass-spectrometry and 16S/rpoA/pheS genes sequencing) and typed by pulsed-field gel electrophoresis (PFGE). The 8 remaining strains belonging to the L. fermentum (6), L. zeae (1) and L. paracasei (1) species were sensitive to all antibiotics tested with the exception of the intrinsic resistance to vancomycin. The strains were all able to grow in artificial saliva. CONCLUSION: Eight strains belonging to L. fermentum, L. zeae and L. paracasei species harbouring anti-elastase and anti-biofilm properties are potential probiotics for fighting P. aeruginosa pulmonary infections. However, further studies are needed in order to test their innocuity and their capacity to behave such as an oropharyngeal barrier against Pseudomonas aeruginosa colonisation in vivo.
Databáze: OpenAIRE
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