High affinity antibody based on a rationally designed hapten and development of a chemiluminescence enzyme immunoassay for quantification of Alternariol in fruit Juice, maize and flour
| Autor: | Yu-Dong Shen, Hong Wang, Chan-Yuan Yao, Fan Zhu, Yuanming Sun, Yuanxin Tian, Zhen-Lin Xu, Lin Luo, Jin-Yi Yang, Hongtao Lei |
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| Rok vydání: | 2017 |
| Předmět: |
Immunogen
Flour Alternariol 01 natural sciences Zea mays Antibodies Analytical Chemistry law.invention Immunoenzyme Techniques chemistry.chemical_compound Lactones 0404 agricultural biotechnology law Limit of Detection Tandem Mass Spectrometry medicine Mycotoxin Chromatography High Pressure Liquid Chemiluminescence Detection limit Chromatography medicine.diagnostic_test Chemistry 010401 analytical chemistry Reproducibility of Results 04 agricultural and veterinary sciences General Medicine Mycotoxins 040401 food science 0104 chemical sciences Fruit and Vegetable Juices Immunoassay Luminescent Measurements Hapten Haptens Food Science Conjugate |
| Zdroj: | Food chemistry. 283 |
| ISSN: | 1873-7072 |
| Popis: | Alternariol (AOH), a secondary metabolites of the genus Alternaria, is a genotoxic mycotoxin. Because the previous "Mannich antigen" for AOH readily underwent unstable induction, a new AOH hapten was designed for inducing a high-quality antibody against AOH using a conjugate of the AOH carboxymethyl derivative with a carrier protein as the immunogen. A competitive indirect chemiluminescence enzyme immunoassay (ciCLEIA) for AOH was optimized and showed good sensitivity (limit of detection 0.068 ng/mL), a linear range (0.11-1.23 ng/mL), and negligible cross-reactivity with analogues. Average rates of recovery from spiked samples (juice and cereal) were between 72.7% and 115.8%, with coefficients of variation14.3%. Results from ciCLEIA correlated well with those of high-performance liquid chromatography coupled with tandem mass spectrometry, meaning the proposed method might be an effective screening tool for detection of AOH in food samples. |
| Databáze: | OpenAIRE |
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