A Protocol for a Novel Human Ex Vivo Model of Aneurysm
| Autor: | Massimo Caputo, Rosaria Bianco, Sarah J George, Jason L. Johnson, Karina Di Gregoli |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
model
General Immunology and Microbiology business.industry General Neuroscience Bristol Heart Institute Bioinformatics medicine.disease Umbilical cord Sudden death General Biochemistry Genetics and Molecular Biology 3Rs medicine.anatomical_structure Aneurysm Human material Aortic aneurysm rupture aneurysm cardiovascular system Medicine business Aortic rupture lcsh:Science (General) Ex vivo lcsh:Q1-390 |
| Zdroj: | STAR Protocols, Vol 1, Iss 3, Pp 100108-(2020) Bianco, R, Di Gregoli, K, Caputo, M, George, S J & Johnson, J L 2020, ' A Protocol for a Novel Human Ex Vivo Model of Aneurysm ', STAR Protocols, vol. 1, no. 3, pp. 100108 . https://doi.org/10.1016/j.xpro.2020.100108 |
| ISSN: | 2666-1667 |
| DOI: | 10.1016/j.xpro.2020.100108 |
| Popis: | Summary Aortic aneurysm rupture is a significant cause of premature mortality worldwide. Although animal models exist, some frequently experience aortic rupture and sudden death. An alternative approach is therefore required that would use human material to aid translation. Accordingly, we present an optimized and validated protocol to isolate human umbilical cord arteries and their subsequent deployment within a bioreactor. Consequently, this reproducible ex vivo human model of aneurysm can be used for pathogenesis studies and accompanying assessment of potential novel therapeutics. |
| Databáze: | OpenAIRE |
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