Rapid identification of stress-related fingerprint from whole bacterial cells of Bifidobacterium lactis using matrix assisted laser desorption/ionization mass spectrometry
| Autor: | Ralf Zink, Laure F. Marvin-Guy, Martin Kussmann, Julie Moulin, Stephan Parche, Laurent B. Fay, Sandrine Wagnière |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Chromatography
Dose-Response Relationship Drug biology Chemistry Analytical chemistry Reproducibility of Results Mass spectrometry biology.organism_classification Proteomics Peptide Mapping Bifidobacterium animalis Bile Acids and Salts Bifidobacteriaceae Matrix-assisted laser desorption/ionization Structural Biology Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Desorption Ionization Mass spectrum Bifidobacterium Spectroscopy |
| Zdroj: | Journal of the American Society for Mass Spectrometry. (8):1222-1227 |
| ISSN: | 1044-0305 |
| DOI: | 10.1016/j.jasms.2004.04.035 |
| Popis: | Whole cells of Bifidobacterium lactis were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). Characteristic and reproducible mass spectra were obtained in the mass range from 6 to 19 kDa. After several days of bacterial cell storage at 4 °C (D0, D2, and D6), only minor signal differences were observed. Under identical and reproducible conditions, fourteen relevant diagnostic ions were identified. Moreover, control- and stress-related fingerprints were rapidly obtained using MALDI-TOFMS by comparison of protein patterns obtained from non-stressed (control) versus stressed cells (addition of bile salts during growth). After quantitative validation of the MALDI-MS data by a statistical approach, two and eight signals were assigned as control- and stress-specific ions, respectively. This work provides the evidence that MALDI-TOFMS can be used for the identification of stress-related fingerprint of B. lactis bacterial cells and could have a high potential for the assessment of the physiological status of the cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|