Biogenic production of cyanide and its application to gold recovery
| Autor: | Gordon A. McFeters, S C Campbell, G J Olson, T R Clark |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Gold mining
Swine Cyanide Microorganism Bioengineering Applied Microbiology and Biotechnology Feces chemistry.chemical_compound Animals Cyanides biology business.industry Chromobacterium Biofilm Industrial microbiology biology.organism_classification Culture Media chemistry Biochemistry Biofilms Glycine Gold Aeration business Chromobacterium violaceum Biotechnology Nuclear chemistry |
| Zdroj: | Journal of Industrial Microbiology and Biotechnology. 26:134-139 |
| ISSN: | 1476-5535 1367-5435 |
| Popis: | is a cyanogenic (cyanide-producing) microorganism. Cyanide is used on an industrial scale to complex and recover gold from ores or concentrates of ores bearing the precious metal. A potentially useful approach in gold mining operations could be to produce cyanide biologically in relatively small quantities at the ore surface. In this study, C. violaceum grown in nutrient broth formed a biofilm and could complex and solubilize 100% of the gold on glass test slides within 4–7 days. Approximately 50% of the cyanide-recoverable gold could be mobilized from a biooxidized sulfidic-ore concentrate. Complexation of cyanide in solution by gold appeared to have a beneficial effect on cell growth — viable cell counts were nearly two orders of magnitude greater in the presence of gold-coated slides or biooxidized ore substrates than in their absence. C. violaceum was cyanogenic when grown in alternative feedstocks. When grown in a mineral salt solution supplemented with 13.3% v/v swine fecal material (SFM), cells exhibited pigmentation and suspended cell concentrations comparable to cultures grown in nutrient broth. Glycine supplements stimulated production of cyanide in 13.3% v/v SFM. In contrast, glycine was inhibitory when added at the time of inoculation in the more concentrated SFM, decreasing cell numbers and reducing ultimate bulk-solution cyanide concentrations. However, aeration and addition of glycine to stationary phase cells grown on 13.3% v/v SFM anaerobically resulted in rapid production and high concentrations (up to 38 mg l−1) of cyanide. This indicates that biogenesis of cyanide may be supported in remote areas using locally produced and inexpensive agricultural feedstocks in place of commercial media. Journal of Industrial Microbiology & Biotechnology (2001) 26, 134–139. |
| Databáze: | OpenAIRE |
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