Human Milk Oligosaccharide 3'-GL Improves Influenza-Specific Vaccination Responsiveness and Immunity after Deoxynivalenol Exposure in Preclinical Models
| Autor: | Toutounchi, Negisa Seyed, Braber, Saskia, Hogenkamp, Astrid, Varasteh, Soheil, Cai, Yang, Wehkamp, Tjalling, Tims, Sebastian, Leusink-Muis, Thea, van Ark, Ingrid, Wiertsema, Selma, Stahl, Bernd, Kraneveld, Aletta D., Garssen, Johan, Folkerts, Gert, Van’T Land, Belinda, Afd Pharmacology, Afd Chemical Biology and Drug Discovery, Pharmacology, Chemical Biology and Drug Discovery |
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| Přispěvatelé: | Afd Pharmacology, Afd Chemical Biology and Drug Discovery, Pharmacology, Chemical Biology and Drug Discovery |
| Rok vydání: | 2021 |
| Předmět: |
Cell
delayed-type hypersensitivity deoxynivalenol Oligosaccharides Ileum Spleen Food Contamination Adaptive Immunity Article immune response mycotoxin Cecum Immune system Immunity Influenza Human medicine Animals Humans TX341-641 Immune response Mycotoxin Vaccines Nutrition and Dietetics Milk Human Nutrition. Foods and food supply business.industry Vaccination Human milk oligosaccharides Mycotoxins Th1 Cells Fatty Acids Volatile Deoxynivalenol In vitro Diet Intestines Mice Inbred C57BL medicine.anatomical_structure Immunology Delayed-type hypersensitiv-ity Female human milk oligosaccharides Caco-2 Cells business Trichothecenes Trisaccharides Food Science |
| Zdroj: | Nutrients Nutrients, 13(9). MDPI AG Nutrients, Vol 13, Iss 3190, p 3190 (2021) Volume 13 Issue 9 |
| ISSN: | 2072-6643 |
| Popis: | Deoxynivalenol (DON), a highly prevalent mycotoxin food contaminant, is known to have immunotoxic effects. In the current study, the potential of dietary interventions with specific mixtures of trans-galactosyl-oligosaccharides (TOS) to alleviate these effects were assessed in a murine influenza vaccination model. Vaccine-specific immune responses were measured in C57Bl/6JOlaHsd mice fed diets containing DON, TOS or a combination, starting 2 weeks before the first vaccination. The direct effects of TOS and its main oligosaccharide, 3′-galactosyl-lactose (3′-GL), on DON-induced damage were studied in Caco-2 cells, as an in vitro model of the intestinal epithelial barrier. Exposure to DON significantly reduced vaccine-specific immune responses and the percentages of Tbet+ Th1 cells and B cells in the spleen. DON significantly altered epithelial structure and integrity in the ileum and reduced the SCFA levels in the cecum. Adding TOS into DON-containing diets significantly improved vaccine-specific immune responses, restored the immune cell balance in the spleen and increased SCFA concentrations in the cecum. Incubating Caco-2 cells with TOS and 3′-GL in vitro further confirmed their protective effects against DON-induced barrier disruption, supporting immune modulation. Overall, dietary intervention with TOS can attenuate the adverse effects of DON on Th1-mediated immune responses and gut homeostasis. These beneficial properties might be linked to the high levels of 3′-GL in TOS. |
| Databáze: | OpenAIRE |
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